[关键词]
[摘要]
目的 通过研究不同比例调和油的抗过敏效果,探讨脂肪酸对过敏性疾病的治疗作用。方法 BALB/c小鼠随机分为对照组、模型组、调和油1(脂肪酸比例为饱和脂肪酸:ω-3:ω-6=1:1:1,5 mg/kg)组、调和油2(脂肪酸比例为饱和脂肪酸:ω-3:ω-6=2:2:1,5 mg/kg)组、地塞米松(阳性对照,5 mg/kg)组,对照组和模型组给予等体积的生理盐水,持续ig 21 d。分别于给药期间的第1、8天,除对照组外,其余小鼠均ip 1 μg/μL鸡卵白蛋白(OVA)的佐剂溶液致敏,每只0.1 mL,对照组ip生理盐水;于给药期间的第15~21天,每天鼻腔滴注10 mg/mL OVA溶液建立过敏性鼻炎模型,隔日ig 250 mg/mL、10 mL/kg的OVA溶液建立肠炎小鼠模型;观察过敏症状并评分;末次激发前2 d,小鼠足垫注射0.05 mLOVA (质量浓度2 mg/mL)溶液,48 h后用足体积记录仪测定每只小鼠左右后足足体积,计算足肿胀率;解剖小鼠,取心脏、脾脏、肾脏、肝脏、胸腺、肺脏、小肠与结肠称质量,计算脏器指数。结果 与模型组比较,调和油1显著降低小鼠过敏性鼻炎评分(P<0.05),但不能显著减少小鼠搔鼻次数;调和油2、地塞米松组小鼠的搔鼻次数均明显下降(P<0.05),但过敏性鼻炎评分显著升高(P<0.05);调和油1和地塞米松均能显著降低小鼠肠道过敏评分和腹泻评分(P<0.05),但调和油2显著增加肠道过敏评分和腹泻评分(P<0.05);调和油1组、调和油2组、地塞米松组小鼠的足肿胀率均显著降低(P<0.05)。与模型组比较,调和油1、2组小鼠体质量显著升高(P<0.05);调和油1组心脏指数显著下降(P<0.05),胸腺指数显著升高(P<0.05);调和油2组心脏指数显著下降,结肠指数显著升高(P<0.05)。地塞米松组小鼠的脾脏、胸腺指数均显著下降,肾脏、肝脏、肺脏指数均显著上升(P<0.05)。结论 调和油1对过敏性鼻炎和肠炎均有良好的抗敏作用,但其止痒效果不佳且会加重胸腺反应;调和油2对过敏性鼻炎和肠炎抗过敏效果不佳同时又会加重肠道过敏症状,但其止痒效果良好且抗炎效果显著。研究结果可指导患者在过敏期选用符合自身病情的调和油食用来改善过敏症状。
[Key word]
[Abstract]
Objective To study the anti-allergic effects of different proportions of blend oil and explore the therapeutic effects of fatty acids on allergic diseases.Methods BALB/c mice were randomly divided into control group,model group,blended oil 1(fatty acid ratio was saturated fatty acid:ω-3:ω-6=1:1:1,5 mg/kg),blend oil 2(fatty acid ratio was saturated fatty acid:ω-3:ω-6=2:2:1,5 mg/kg),and dexamethasone (positive control,5 mg/kg) group.The mice in control and model group were given the same volume of physiological saline,and corresponding drugs were ig given to mice for 21 d continuously.On the 1st and 8th days of administration,except the control group,all mice in the other groups were sensitized by ip 100 μg OVA/100 μL adjuvant solution,0.1 mL for each mouse,and ip saline for control group.The allergic rhinitis model was established by intranasal drip of 10 mg/mL OVA solution on day 15-21 during administration,and the enteritis mice model was established by using ig 250 mg/mL,10 mL/kg OVA solution every other day.The allergic symptoms were observed and graded.Two days before the last stimulation,0.05 mL OVA (2 mg/mL) solution was injected into the footpad of mice,and 48 h later,the foot volume of each mouse was measured by foot volume recorder to calculate the foot swelling rate;The heart,spleen,kidney,liver,thymus,lung,small intestine,and colon were dissected and weighed,and the organ index was calculated.Results Compared with the model group,blend oil 1 significantly decreased the allergic rhinitis score (P< 0.05),but could not significantly reduce the times of scratching nose in mice;Blending oil 2 and dexamethasone significantly decreased the times of scratching nose in mice (P< 0.05),but the allergic rhinitis score was significantly increased (P< 0.05);Blending oil 1 and dexamethasone significantly reduced the intestinal allergy score and diarrhea score in mice (P< 0.05).However,the intestinal allergy score and diarrhea score were significantly increased by blending oil 2(P< 0.05);The foot swelling rate of mice in blending oil 1 group,blending oil 2 group,and dexamethasone group were significantly decreased (P< 0.05).Compared with the model group,the body mass of mice in the blended oil group 1 and 2 was increased significantly (P< 0.05);The heart index and thymus index in the blended oil group 1 were decreased significantly (P< 0.05);The heart index was significantly decreased and colon index was significantly increased in the blended oil group 2(P< 0.05).The spleen and thymus index of mice in dexamethasone group were decreased significantly,while the kidney,liver and lung index were increased significantly (P< 0.05).Conclusion Blend oil 1 has significant antiallergic effects on allergic rhinitis and enteritis,but its antipruritic effect was unsatisfactory and the risk of thymus reaction was increased;Blend oil 2 had an unsatisfactory effect on allergic rhinitis and enteritis,and it could increase the intestinal tract allergy symptoms,but it had good antipruritic and antiinflammatory effects.Thus,this study could guide patients to choose suitable blend oil to alleviate allergy symptoms by their own conditions during the allergic period.
[中图分类号]
[基金项目]