目的 研究西黄丸组分中药是否与西黄丸具有相似的抗癌作用及机制。方法 4T1乳腺癌细胞荷瘤建立动物模型，西黄丸低、中、高剂量（0.39、0.78、1.95 g/kg）和西黄丸组分中药0.3 mL （74.5 mg/kg榄香烯、2.73 mg/kg牛磺酸、0.52mg/kg麝香酮和4.75 mg/kg 11-羰基-β乙酯乳香酸混合物，对应于西黄丸高剂量组） ig给药14 d，剥离肿瘤组织，称质量，匀浆，制备单细胞悬液，免疫磁硃分离Treg细胞。流式细胞术和免疫组化检测肿瘤微环境中Treg细胞数量变化情况，Western Blotting检测肿瘤微环境中Treg细胞磷脂酰肌醇3-激酶/蛋白激酶B （PI3K/AKT）蛋白表达情况，实时荧光定量PCR （qRT-PCR）检测肿瘤微环境中Treg细胞PI3K、AKT mRNA表达情况。结果 与模型组及西黄丸低、中剂量组比较，西黄丸组分中药组肿瘤质量显著减少（P<0.05）；流式细胞术和免疫组化结果显示，肿瘤微环境中Treg细胞数量显著减少（P<0.05）；Western Blotting和qRT-PCR结果显示，肿瘤微环境中Treg细胞PI3K、AKT蛋白及mRNA表达显著下降（P<0.05）。与西黄丸高剂量组比较，西黄丸组分中药组肿瘤质量、Treg细胞数量和PI3K、AKT蛋白及mRNA表达均明显升高（P<0.05）。结论 西黄丸组分中药通过抑制肿瘤微环境中Treg细胞PI3K/AKT信号通路的表达减少Treg细胞数量，进而表现与西黄丸相似的抗癌作用和机制。

Objective To study whether the Xihuang Pills component-based Chinese medicine has similar anti-cancer effect and mechanism as Xihuang Pills.Methods 4T1 breast cancer cells were used to establish a tumor model in mice.The model mice were ig administered with Xihuang Pills of low,medium,and high doses (0.39,0.78 and 1.95 g/kg) and Xihuang Pills component-based Chinese medicine of 0.3 mL (74.5 mg/kg elemene,2.73 mg/kg taurine,0.52 mg/kg Muscone and 4.75 mg/kg 11-carbonyl-beta-ethyl ester mastic acid mixture,corresponding to the high dosage group of Xihuang Pills) for 14 d.The tumor tissues were removed,weighed and homogenized to prepare single cell suspensions.Treg cells were prepared by magnetic cell sorting.The changes in number of Treg cells in the tumor microenvironment were detected by flow cytometry and immunohistochemistry.The protein expression of PI3K and AKT in Treg cells in the tumor microenvironment was detected by western blotting.The mRNA expression of PI3K and AKT in Treg cells in the tumor microenvironment was detected by the Real-time PCR (qRT-PCR).Results Compared with model group and Xihuang Pills low-and medium-dose groups,the weight of tumors was significantly decreased in the Xihuang Pills component-based Chinese medicine group (P< 0.05);The results of flow cytometry and immunohistochemistry showed a significant decrease in the number of Treg cells in the tumor microenvironment (P< 0.05);Western Blotting and qRT-PCR results showed that the expression of PI3K,AKT protein and their mRNA in Treg cells in the tumor microenvironment was significantly decreased (P< 0.05).Compared with the Xihuang Pills high-dose group,the tumor weight,the number of Treg cells,and the protein expression of PI3K and AKT and their mRNA expression in the Xihuang Pills component-based Chinese medicine group were significant increased (P< 0.05).Conclusion Xihuang Pills component-based Chinese medicine can reduce the number of Treg cells by inhibiting the expression of PI3K/AKT signaling pathway in Treg cells in the tumor microenvironment,and thus exhibit similar anti-cancer effects and mechanisms as Xihuang Pills.

国家自然科学基金资助项目（81573664）