[关键词]
[摘要]
目的 建立高效液相色谱法(HPLC)同时测定附子山茱萸联合给药后大鼠心肌组织中三磷酸腺苷(ATP)、二磷酸腺苷(ADP)、一磷酸腺苷(AMP)、磷酸肌酸(PCr)和肌酐5种物质含量的方法,用于慢性心衰大鼠心肌组织样品能量物质的测定。方法 考察色谱条件(色谱柱、流动相、柱温、检测波长、进样量、洗脱梯度和洗脱时间等);评价该方法的精密度、线性和回收率、检测限与定量限、稳定性等。结果 专属性、线性、精密度、回收率、稳定性均良好,能满足后续研究的需要。动物给药后能量物质均有不同程度升高,且联合给药效果更显著。结论 方法检测结果可靠、操作简便;检测方法可用于慢性心衰大鼠心肌组织样品能量物质的测定。
[Key word]
[Abstract]
Objective Establish a method for the simultaneous determination of five substances (ATP, ADP, AMP, PCr and creatinine) in myocardium of rats by high performance liquid chromatography (HPLC). Methods The chromatographic conditions (column, mobile phase, column temperature, detection wavelength, injection volume, elution gradient and elution time) were investigated. The method was evaluated for precision, linearity and recovery, limit of detection and limit of quantization, stability, and so on.Results Specificity, linearity, precision, recovery and stability were all good, which could meet the need of follow-up study. After administration, the energy and substance of animals all have different degrees of increase.Conclusion The method is reliable and easy to operate. The detection method can be used for the determination of energy substances in myocardial tissue of chronic heart failure rats.
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[基金项目]
广东省自然科学基金(N0.2016A030313646);广东省科技计划项目(广东省实验动物与动物实验技术综合创新平台建设(一期),2017A070702001)