[关键词]
[摘要]
目的 建立橘叶UPLC指纹图谱,对不同产地药材进行质量评价,为科学评价各批次橘叶质量提供依据。方法 采用Acquty UPLC BEH C18(100 mm×2.1 mm,1.7 μm)色谱柱,以0.2%甲酸水溶液-甲醇为流动相进行梯度洗脱,体积流量为0.3 mL/min,柱温为26℃,检测波长为330 nm。结合聚类分析和主成分分析对20批不同产地橘叶药材的整体质量进行评价。结果 建立20批橘叶的UPLC对照指纹图谱,标定27个共有峰,聚类分析分成4类,利用主成分分析进行验证且确定6个决定峰。结论 结合化学计量学和UPLC指纹图谱为建立全面有效的橘叶药材质量控制模式提供参考。
[Key word]
[Abstract]
Objective To establish fingerprint of Folium Citri Reticulatae by UPLC, and assess the quality of different habitats, provide reasonable reference for its quality evaluation and control. Methods The ACQUTY UPLC BEH C18 (100 mm×2.1 mm, 1.7 μm) was used with a mobile phase of methanol-0.2% pheosphoric acid in gradient elution. The flow rate was 0.3 mL/min. The column temperature was 26℃, and detection wavelength was 330 nm. Principal component analysis and cluster analysis were used to evaluate 20 batches quality of Folium Citri Reticulatae from different habitats. Results Fingerprints of 20 batches of samples were established and 27 common peaks were obtained. All of samples could be classified into four cluste by cluster analysis, and PCA was used to verify and identify 6 determination peaks. Conclusion The combination of Chemometrics (CMM) and fingerprint can provide a reference for the comprehensive and effective quality control of herbs.
[中图分类号]
[基金项目]
国家重大科技专项重大新药创制——中医药优势领域的创新中药关键技术开发研究(2017ZX09301005)