[关键词]
[摘要]
目的 通过对金芪降糖片血清药物化学研究方法的建立,探讨其口服吸收后的入血成分。方法 利用超高效液相色谱-电喷雾串联四级杆质谱(UPLC-ESI-MS)和多重反应监测(MRM)对金芪降糖片样品及ig给予金芪降糖片后大鼠血清样品进行分析,比较各样品分析结果,确认金芪降糖片的血中移行成分。结果 ig给予金芪降糖片后大鼠血清中发现16种原形产物,分别为新绿原酸、绿原酸、隐绿原酸、咖啡酸、木兰花碱、芦丁、金丝桃苷、木犀草苷、异绿原酸A、异绿原酸C、黄连碱、表小檗碱、药根碱、小檗碱、巴马汀、黄芪甲苷。结论 确定了金芪降糖片的血中移行成分,为阐明其有效成分及作用机制提供参考。
[Key word]
[Abstract]
Objective To explore the absorbed constituents in plasma after oral administration of Jinqi Jiangtang Tablets determined by its serum pharmacochemistry research method. Methods Using UPLC-ESI-MS and multiple response monitoring (MRM) method, the chromatograms of rat serum samples after oral administration of Jinqi Jiangtang Tablets were analyzed and compared with samples of rats in the control group, in order to identify the constituents absorbed into blood of Jinqi Jiangtang Tablets. Results A total of 16 original form compounds absorbed into the blood after ig administration of Jinqi Jiangtang Tablet were detected, including Neochlorogenic acid, Chlorogenic acid, Cryptochlorogenic acid, Caffeic acid, Magnoflorine, Rutin, Hyperoside, Luteoloside, Isochlorogenic acid A, Isochlorogenic acid C, Coptisine, Epiberberine, Jatrorrhizine, Berberine Palmatine, and AstragalosideA. Conclusion The constituents absorbed into blood after oral administration of Jinqi Jiangtang Tablets were identified in this study, which is conductive to the exploration of bioactive constituents of Jinqi Jiangtang Tablets and its mechanism of action.
[中图分类号]
[基金项目]
国家自然科学基金项目(21502142);天津市应用基础与前沿技术研究计划(15JCQNJC14100)