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[摘要]
目的 探讨熄风静宁颗粒(XFJNG)对多发性抽动症(TS)模型大鼠HPA轴及纹状体单胺类神经递质的影响。方法 采用连续7 d ip亚氨基二丙腈(IDPN,150 mg/kg)建立TS大鼠模型,造模成功后将大鼠随机分为模型组、氟哌啶醇(0.5 mg/kg)组、XFJNG(6 mg/kg,临床等效剂量)组,同时设立对照组,每组10只,连续给药4周。于给药第0、1、2、3、4周进行刻板运动评分;末次给药后24 h杀鼠取材,试剂盒法检测各组大鼠纹状体内多巴胺(DA)、去甲肾上腺素(NE)、5-羟色胺(5-HT)含量,下丘脑及血清促肾上腺皮质素释放激素(CRH)含量,血清促肾上腺皮质激素(ACTH)、皮质醇(CORT)含量;取肾上腺组织,进行HE染色,光学显微镜下观察大鼠肾上腺组织形态。结果 与模型组比较,给药4周后,氟哌啶醇和XFJNG组大鼠刻板运动评分均显著降低,纹状体DA、NE、5-HT的水平均显著升高,血清及下丘脑CRH水平均显著降低,血清ACTH及CORT水平均显著降低,差异均具有统计学意义(P<0.05)。与对照组比较,模型组大鼠肾上腺细胞体积较小,染色深,细胞质占比较大,空泡明显减少;氟哌啶醇、XFJNG组大鼠肾上腺细胞质染色相对模型组较浅,空泡数量增多。结论 熄风静宁颗粒可能通过抑制大鼠纹状体DA系统失衡及HPA轴功能亢进来改善大鼠TS症状。
[Key word]
[Abstract]
Objective To explore the effect of Xifeng Jingning Granule (XFJNG) on the HPA Axis and striatum monoamine neurotransmitters in tics Tourette syndrome (TS) rats.Methods TS model was induced by ig injection of iminodipropionitrile (IDPN, 150 mg/kg), then TS rats were randomly divided into model group, Haloperidol group (0.5 mg/kg), XFJNG group (6 mg/kg) and meanwhile establish control group, each group was 10 cases. Then the rats were treated for 4 weeks and the stereotyped movement scores were recorded in the 0, 1th, 2th, 3th, 4th week. The striatum levels of DA, NE, 5-HT, the hypothalamus and serum levels of CRH, the serum levels of ACTH and CORT were measured by kits. The adrenal gland tissues were stained with HE and the morphology of adrenal glands was observed under light microscope. Results Compared with the model group, the stereotyped movement scores of the rats in the medicine group and traditional medicine group were significantly decreased, the striatum levels of DA, NE, 5-HT, the hypothalamus and serum levels of CRH, the serum levels of ACTH and CORT in the medicine group and traditional medicine group were significantly decreased, all above had statistical difference (P<0.05). Compared with the control group, the adrenal cells in the model group were smaller, stained deeply, the proportion of cytoplasm was larger, and the number of vacuoles was significantly reduced. In the haloperidol group and XFJNG group, the adrenal gland cytoplasm staining was relatively shallow and the number of vacuoles increased. Conclusion XFJNG could improve TS by inhibiting DA system imbalance and HPA axis hyperfunction.
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[基金项目]
高等学校博士学科点专项科研基金项目(20120013120011)