目的 建立不同产地土贝母药材中皂苷类成分的特征图谱，并测定其中3种主要皂苷的含量，为该药材质量控制提供参考依据。方法 采用Thermo Hypersil GOLD aQ C18色谱柱（250 mm×4.6 mm，5 μm），乙腈-水为流动相进行梯度洗脱，体积流量1.0 mL/min，进样量10 μL，柱温30℃，检测波长214 nm，建立15批土贝母HPLC特征图谱，并进行相似度评价、主成分分析及聚类分析；采用Diamonsil C18色谱柱（250 mm×4.6 mm，5 μm），以乙腈-水为流动相进行等度洗脱测定药材中3种皂苷类成分的含量。结果 建立的15批土贝母特征图谱共标定6个共有峰，相似度为0.845～0.98；主成分分析及聚类分析均将15批药材分为两类，与相似度评价结果一致；得到影响药材质量的3个皂苷成分（土贝母苷甲、乙、丙）并对其进行了含量测定，结果 15批药材都符合药典含量限度的要求。结论 本研究所建立的HPLC特征图谱结合含量测定以及相似度评价，主成分分析，聚类分析等评价方法，可以全面、准确和有效地用于土贝母药材的质量评价。
Objective To establish the HPLC characteristic spectrum of Bolbostemma paniculatum from different areas, determine the contents of three saponinsa, and provide basis for quality control of B. paniculatum. Methods For characteristic spectrum, chromatographic experiments were performed on an Thermo Hypersil GOLD aQ C18 column (250 mm×4.6 mm, 5 μm) using gradient elution with acetonitrile-aqueous as the mobile phase at a flow rate of 1.0 mL/min, the detection wavelength was 214 nm, the sample size was 10 μL, and the column temperature was set at 30℃. In the end, similarity evaluation, principal component analysis and cluster analysis were carried out. An HPLC method for quantitative analysis was applied with an Diamonsil C18 column (250 mm×4.6 mm, 5 μm) using isocratic elution with acetonitrile-aqueous as the mobile phase at a flow rate of 1.0 mL·min-1, the detection wavelength was 214 nm, the sample size was 10 μL, and the column temperature was set at 30℃.Results There were 6 common peaks identified in the characteristic spectra of 15 batches of B. paniculatum, and the similarity was between 0.845-0.98. All batches of samples can be classified into two groups by using PCA and HCA, which were in accordance with the results of similarity evaluation. The three key components (tubeimoside I, Ⅱ and Ⅲ) affecting the quality of the medicinal materials were identified and their contents were determined.Conclusion The HPLC characteristic spectrum, similarity evaluation, PCA, CA and content determination method can comprehensively, accurately and effectively evaluate the quality of B. paniculatum.