目的 探究健脾祛湿方（JPQS）抗肥胖作用及对脂质代谢和慢性炎症的影响。方法 56只雄性SD大鼠适应性饲养1周后，随机分为对照组、模型组、奥利司他（32.4 mg·kg^-1）组及JPQS高、低剂量（0.70、0.35 g·kg^-1）组。对照组给予纯化饲料，其余各组给予高脂饲料喂养，持续8周，建立单纯性肥胖大鼠模型。造模同时ig给药干预，每天1次，对照组和模型组每日ig给予蒸馏水，连续给药8周。末次给药后，测定大鼠体质量、内脏白色脂肪含量、脂体比、Lee’s指数等。采用酶联免疫法（ELISA）检测各组大鼠血清中脂联素（ADP）、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）的水平；采用实时荧光定量PCR（qRT-PCR）技术检测各组大鼠附睾旁脂肪的脂肪酸合成酶（FAS）、脂肪三酰甘油水解酶（ATGL）和脂蛋白脂肪酶（LPL）的mRNA相对表达量；采用苏木精-伊红（HE）染色法观察各组大鼠肝脏及附睾旁白色脂肪组织的形态；采用油红O染色法观察各组大鼠肝脏脂滴沉积情况。结果 与对照组比较，模型组大鼠的体质量、体质量增重、Lee’s指数、内脏脂肪含量及脂体比均显著升高（P＜0.05、0.01、0.001）； ADP含量显著降低（P＜0.05），TNF-α和IL-6含量显著升高（P＜0.01）； FAS mRNA水平显著升高（P＜0.05），ATGL和LPL mRNA水平显著降低（P＜0.05）。与模型组相比，JPQS高、低剂量组大鼠的体质量、体质量增重、Lee’s指数、内脏脂肪含量及脂体比均显著降低（P＜0.05、0.01、0.001），摄食量无明显影响； ADP含量显著增多（P＜0.05），TNF-α和IL-6含量显著降低（P＜0.01、0.001）； FAS mRNA水平显著减少（P＜0.01），ATGL和LPL mRNA水平显著升高（P＜0.05、0.01、0.001）。结论 JPQS可以通过抑制体质量及内脏脂肪含量的增长进而预防肥胖的发生发展，其机制可能与改善脂质代谢及缓解慢性炎症有关。

Objective To explore the anti-obesity effect of Jianpi-Qushi Prescription (JPQS) on simple obese rats and its effects on lipid metabolism and chronic inflammation. Methods After one week of adaptive feeding, 56 male SD rats were randomly divided into the control group, the model group, the orlistat (32.4 mg·kg^-1) group and the high-dose (0.70 g·kg^-1) and low-dose (0.35 g·kg^-1) JPQS groups. The control group was fed with purified feed, while the other groups were fed with high-fat feed for eight weeks to establish a simple obesity rat model. At the same time of modeling, ig administration was given once a day. The control group and the model group were ig administered with distilled water daily for eight consecutive weeks. After the last administration, the weight, visceral white fat content, fat-body ratio, Lee's index and other indicators were measured. The levels of serum adiponectin (ADP), tumor necrosis factor-α (TNF-α) and interleukin -6 (IL-6) were detected by enzyme-linked immunosorbent assay (ELISA). The relative mRNA expression of fatty acid synthase (FAS), adipose triglyceride lipase (ATGL) and lipoprotein lipase (LPL) in epididymal fat of rats in each group was detected by real-time fluorescence quantitative PCR (qRT-PCR). Hematoxylin-eosin (HE) staining was used to observe the morphology of liver and white adipose tissue of rats in each group. Oil red O staining was used to observe the deposition of lipid droplet of liver. Results Compared with the control group, the body weight, weight gain, Lee's index, visceral fat content and fat-body ratio of rats in model group were significantly decreased (P <0.05, 0.01, 0.001). The level of ADP increased significantly (P < 0.05), while the level of TNF-α and IL-6 decreased significantly (P < 0.01); The relative mRNA expression of FAS was downregluated significantly (P < 0.05), while the relative mRNA expression of ATGL and LPL were up-regluated significantly (P < 0.05). Compared with the model group, the body weight, weight gain, Lee's index, visceral fat content and fat-body ratio of rats in the high and low dose groups of JPQS were significantly decreased (P < 0.05, 0.01, 0.001), but the food intake had no obvious effect. The level of ADP increased significantly (P < 0.05), while the level of TNF-α and IL-6 decreased significantly (P < 0.01); The relative mRNA expression of FAS down-regluated significantly (P < 0.01), while the relative mRNA expression of ATGL and LPL up-regluated significantly (P < 0.05, 0.01, 0.001). Conclusion JPQS could prevent obesity by inhibiting the growth of body weight and visceral fat content, and its mechanism may be related to improving lipid metabolism and relieving chronic inflammation.

R285.5

国家重点研发计划项目（2018YFC1706804）