目的 建立不同颜色怀菊花HPLC指纹图谱与多成分含量测定方法，为其质量控制提供科学依据。方法 建立30批不同颜色怀菊花样品的HPLC指纹图谱，并进行相似度评价，结合化学计量学分析，对多指标成分进行含量测定。结果 建立的指纹图谱共标定21个共有峰，成功指认出10个色谱峰，30批样品的指纹图谱相似度为0.955～0.997。聚类分析（HCA）与主成分分析（PCA）可将样品明确分为怀黄菊、怀白菊、怀紫菊3个类别，实现基于化学成分特征的颜色分型。偏最小二乘法-判别分析（PLS-DA）进一步筛选出9号峰（异绿原酸B）、11号峰（异绿原酸C）、10号峰（异绿原酸A）、3号峰、21号峰（金合欢素）、14号峰、18号峰、16号峰和15号峰（木犀草素）所代表的成分是区分不同批次样品的差异性标志物。30批样品中5种酮类成分与7种酚酸类成分的质量分数总值依次为怀白菊＞怀黄菊＞怀紫菊。结论 建立的HPLC指纹图谱结合多成分含量测定方法具有准确性高、稳定性好、可靠性强的特点，可全面反映不同颜色怀菊花的化学成分差异与整体质量特征，为其质量评价、标准制定及合理开发应用提供有力支撑。

Objective To establish HPLC fingerprint chromatograms and multi-component content determination methods for different-colored Huai Chrysanthemums morifolium, providing a scientific basis for their quality control. Methods HPLC fingerprint chromatograms of 30 batches of different-colored Huai C. morifolium samples were established and similarity evaluation was conducted. Chemometrics analysis was combined, and the contents of multiple index components were determined. Results A total of 21 common peaks were calibrated in the established fingerprint chromatograms, 10 chromatographic peaks were successfully identified. The similarity of the fingerprint chromatograms of the 30 batches of samples was 0.955 to 0.997. Hierarchical cluster analysis (HCA) and principal component analysis (PCA) could clearly classify the samples into three categories: Huai C. morifolium (yellow, white and purple) achieving color classification based on chemical component characteristics. Partial least squaresdiscriminant analysis (PLS-DA) further screened out components represented by peak 9 (isochlorogenic acid B), peak 11 (isochlorogenic acid C), peak 10 (isochlorogenic acid A), peak 3, peak 21 (acacetin), peak 14, peak 18, peak 16, and peak 15 (luteolin) as the differential markers for distinguishing different batches of samples. The total mass fractions of five flavonoids and seven phenolic acids in the 30 batches of samples were in the order of Huai white C. morifolium > Huai yellow C. morifolium > Huai purple C. morifolium. Conclusion The HPLC fingerprint chromatogram combined with multi-component content determination method established in this study has the characteristics of high accuracy, good stability, and strong reliability. It can comprehensively reflect the chemical component differences and overall quality characteristics of different-colored Huai C. morifolium, providing strong support for their quality evaluation, standard formulation, and rational development and application.

R284.2

河南省科技公关项目（242102310552）；国家中医药管理局全国老药工传承工作室项目（国中医药人教函[2025]181号）