目的 研究发酵松花粉对肠道损伤的治疗作用。方法 分别构建葡聚糖硫酸钠（DSS）诱导的小鼠肠道损伤模型和吲哚美辛诱导的小鼠肠道损伤模型，造模后24 h均以ig方式给予1.5、3.0 g·kg^-1的松花粉（PM）和发酵松花粉（FPM）14 d，以0.6 g·kg^-1柳氮磺吡啶肠溶片为阳性药。分别记录2种不同造模方式小鼠体质量变化，计算肝和脾的脏器指数；测定结肠长度；采用苏木精-伊红（HE）染色法和马松染色（Masson）对小鼠肠道进行组织病理学观察；酶联免疫吸附法测定血浆和肠组织中细胞因子的水平变化。结果 在DSS和吲哚美辛诱导的2种小鼠肠道损伤模型中，肠道组织炎症浸润严重，其细胞排列混乱；与对照组相比，模型组小鼠体质量显著减轻，结肠组织病理评分显著增加，结肠长度显著降低，肝脏指数和脾脏指数显著增加，小鼠血浆中超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的水平显著降低，结肠组织细胞因子肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6、IL-12、免疫球蛋白A（IgA）的含量显著增加，IL-10的含量显著降低，差异均显著（P＜0.01）。与模型组相比，PM高剂量和FPM低、高剂量组小鼠肠道损伤状况减轻，病理评分显著降低，体质量变化率增加，肝脏肿大减轻，结肠长度增加，小鼠血浆中SOD和GSH-Px的含量升高，细胞因子TNF-α、IL-6、IL-12及IgA的含量降低，IL-10的含量升高； FPM高剂量组脾脏肿大减轻，差异均显著（P＜0.05、0.01）。与等剂量的PM比较，FPM组在体质量变化率、病理评分、脏器指数方面均作用更显著（P＜0.05、0.01）。结论 PM和FPM可改善小鼠的肠道损伤状况，且经发酵后的PM效果优于未发酵的PM。

Objective To investigate the protective and therapeutic effects of fermented Pinus massoniana (FPM) on intestinal injury. Methods Dextran sulfate sodium (DSS)-induced and indomethacin-induced mouse models of intestinal injury were established. After 24 h of modeling, 1.5 and 3.0 g·kg^-1 of pine pollen (PM) and fermented pine pollen (FPM) were administered ig for 14 days, with 0.6 g·kg^-1 sulfasalazine enteric-coated tablets as the positive drug. The changes in body weight of mice in the two different models were recorded, and the organ indices of the liver and spleen were measured. The colon length was measured. The intestinal tissues of mice were observed histopathologically by hematoxylin-eosin staining (HE) and Masson staining. The levels of cytokines in plasma and intestinal tissues were determined by enzyme-linked immunosorbent assay. Results In the two mouse models of intestinal injury induced by DSS and indomethacin, the intestinal tissue showed severe inflammatory infiltration and disordered cell arrangement. Compared with the control group, the model group showed significant weight loss, significantly increased colonic histopathological scores, significantly reduced colon length, significantly increased liver and spleen coefficients, significantly decreased levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in plasma, and significantly increased levels of cytokines TNF-α, IL-6, IL-12, and IgA in colonic tissues, and significantly decreased levels of IL-10, with significant differences (P < 0.01). Compared with the model group, the high-dose PM and low- and high-dose FPM groups showed alleviated intestinal injury, significantly reduced histopathological scores, increased body weight change rates, reduced liver enlargement, increased colon length, increased levels of SOD and GSH-Px in plasma, decreased levels of cytokines TNF-α, IL-6, IL-12, and IgA, and increased levels of IL-10; the highdose FPM group showed reduced spleen enlargement, with significant differences (P < 0.05, 0.01). Compared with the same dose of PM, the FPM group showed more significant effects in body weight change rate, histopathological score, and organ coefficie nt (P < 0.05, 0.01). Conclusion PM and FPM can improve intestinal injury in mice with colitis, and FPM exhibits better efficacy than unfermented PM.

R285.5

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