目的 结合化学与生物评价指标，采用层次分析（AHP）-基于指标相关性的权重确定方法（CRITIC）复合加权优选杧果叶提取工艺，并对其促消化活性进行评价，为杧果叶药用开发提供实验依据。方法 建立杧果叶提取物中多酚、黄酮含量和胰脂肪酶抑制活性的分析方法；以乙醇体积分数、提取温度、料液比、提取时间为考察因素，筛选关键工艺参数；分别运用AHP法、CRITIC法及AHP-CRITIC混合加权法确定各指标权重，结合Box-Behnken响应面（BBD-RSM）法优化提取工艺，并测定提取物对胰脂肪酶的半数抑制浓度（IC₅₀）；构建大鼠胃肠积滞模型，从胃排空率、小肠推进率、胃动素含量及胃组织病理形态方面评价提取物体内促消化作用。结果 乙醇体积分数对各项评价指标影响最为显著； AHP-CRITIC混合加权法兼顾主观与客观赋权优势，所建响应面模型拟合效果良好。确定最优提取工艺为乙醇体积分数60%、提取温度70℃、料液比1∶ 18。该工艺所得提取物对胰脂肪酶的半数抑制浓度（IC₅₀）为（5.022± 0.006） mg·mL^-1；动物实验表明，杧果叶提取物可显著提升模型大鼠胃排空率、小肠推进率与胃动素含量，同时修复受损胃组织。结论 AHP-CRITIC混合加权法联合BBD-RSM法可有效优化杧果叶提取工艺，所得提取物具备良好的体外胰脂肪酶抑制活性与体内促消化作用，可为杧果叶的质量研究、资源化利用及促消化相关产品研发提供参考。

Objective To optimize the extraction process of mango Mangifera indica extract using a combined weighting method integrating Analytic Hierarchy Process (AHP) and Criteria Importance Through Inter-criteria Correlation (CRITIC), based on chemical and biological evaluation indicators, and to evaluate its digestive-promoting effect, thereby providing experimental support for the medicinal development of M. indica leaves. Methods An analytical method was established to determine polyphenol and flavonoid contents and pancreatic lipase inhibitory activity in M. indica leaves extracts. Ethanol concentration, extraction temperature, solidliquid ratio, and extraction time were selected as key process variables. The weights of each indicator were determined separately by AHP, CRITIC, and the hybrid AHP-CRITIC weighting method, and the extraction process was optimized using Box-Behnken designresponse surface methodology (BBD-RSM). A rat model of gastrointestinal stagnation was constructed to evaluate the in vivo motilitypromoting effects of the extract through gastric emptying rate, small intestinal transit rate, motilin levels, and histopathological changes in gastric tissue. The half-maximal inhibitory concentration (IC₅₀) of the extract against pancreatic lipase was also measured. Results Ethanol concentration had the most significant effect on all evaluated parameters. The AHP-CRITIC hybrid weighting method effectively combined subjective and objective weighting advantages, and the resulting response surface model showed excellent fitting performance. The optimal extraction conditions were identified as 60% ethanol concentration, 70 ℃ extraction temperature, and a solid-liquid ratio of 1∶ 18. The IC₅₀ value of the extract against pancreatic lipase under these conditions was (5.022 ± 0.006) mg·mL^-1. Animal experiments demonstrated that M. indica leaves extract significantly improved gastric emptying rate, small intestinal transit rate, and motilin levels in model rats, while also repairing damaged gastric tissues. Conclusion The combination of AHP-CRITIC hybrid weighting with BBD-RSM is effective in optimizing the M. indica leaves extraction process. The obtained extract exhibits strong in vitro inhibition of pancreatic lipase and significant in vivo gastrointestinal motility-promoting effects, offering valuable insights for quality research, resource utilization, and the development of digestive-promoting products from M. indica leaves.

R285.5

广西自然科学基金项目（2024GXNSFBA010168，2025GXNSFAA069493）；大学生创新训练计划项目（S202410600111）；广西中药药效研究重点实验室项目（桂科计字〔 2005〕 6号）；农作物废弃物功能成分研究协同创新中心资助课题（CICAR2023-P1）