目的 建立蒙荨麻的HPLC特征图谱，结合化学计量学分析、体外实验验证及含量测定，筛选其抗高尿酸血症的质量标志物（Q-Marker）。方法 采用HPLC法建立15批蒙荨麻的特征图谱；结合层次聚类分析（HCA）、主成分分析（PCA）及偏最小二乘法-判别分析（PLS-DA）筛选不同产地样品的差异成分；利用Discovery studio 2016 Client软件将潜在Q-Marker与抗高尿酸血症相关靶蛋白[黄嘌呤氧化酶（XOD）、葡萄糖转运蛋白9（GLUT9）]进行分子对接验证；通过体外XOD酶活力抑制实验及HK2细胞GLUT9蛋白表达检测验证其生物活性；并建立目标成分含量测定方法，测定15批次样品中QMarker的含量。结果 从HPLC特征图谱中指认绿原酸、新西兰牡荆苷2、异牡荆苷3个特征成分；化学计量学分析将15批次样品分为2类，其中新西兰牡荆苷2和异牡荆苷对质量差异的贡献最显著；分子对接显示，指认的3个特征成分均能通过氢键、疏水键、π-π键等与XOD、GLUT9稳定结合，绿原酸与GLUT9结合能最低(-36.00 kJ·mol^-1)，新西兰牡荆苷2与XOD结合能最低(-45.61 kJ·mol^-1)；体外实验证实，3个成分均呈浓度相关性地抑制XOD活力，且不同浓度给药组可显著下调HK2细胞GLUT9蛋白表达（P<0.01）；含量测定显示，15批次样品中绿原酸、新西兰牡荆苷2、异牡荆苷的质量分数分别为0.144 3～4.234 6、0.118 6～2.581 2、0.056 9～3.670 0 mg·g^-1。结论 建立的HPLC特征图谱及含量测定方法准确、可靠，绿原酸、新西兰牡荆苷2、异牡荆苷可作为蒙荨麻抗高尿酸血症的Q-Marker，为其药材质量评价与控制提供依据。

Objective To establish the HPLC characteristic fingerprint of mongolian medicine Urtica cannabina, combined with chemometrics analysis, in vitro experiments, and content determination, to screen the quality markers（Q-Markers） for its antihyperuricemia effect. Methods The HPLC method was used to establish the characteristic fingerprint of 15 batches of U. cannabina; hierarchical cluster analysis（HCA）, principal component analysis（PCA）, and partial least squares-discriminant analysis（PLS-DA） were used to screen the different components of samples from different origins; the potential Q-Markers was verified by molecular docking with anti-hyperuricemia related target proteins [xanthine oxidase（XOD）, glucose transporter 9（GLUT9）]; in vitro XOD enzyme activity inhibition experiments and HK2 cell GLUT9 protein expression detection were used to verify its biological activity; and the content determination method of the target components was established to determine the content of Q-Markers in 15 batches of samples. Results Three characteristic components, namely chlorogenic acid, vicenin-2, and isovitexin, were identified from the HPLC characteristic fingerprint; chemometrics analysis divided the 15 batches of samples into two categories, among which vicenin-2, and isovitexin had the most significant contribution to the quality differences; molecular docking showed that the three identified characteristic components could stably bind to XOD and GLUT9 through hydrogen bonds, hydrophobic bonds, and π-π bonds, with the binding energy of chlorogenic acid being the lowest(-36.00 kJ·mol^-1), and that of vicenin-2 to XOD being the lowest(-45.61 kJ·mol^-1); in vitro experiments confirmed that all three components inhibited XOD activity in a concentrationdependent manner, and different concentration administration groups could significantly down-regulate the expression of GLUT9 protein in HK2 cells（P < 0.01）; content determination showed that the quality fractions of chlorogenic acid, vicenin-2, and isovitexin in 15 batches of samples were 0.144 3—4.234 6, 0.118 6—2.581 2, and 0.056 9—3.670 0 mg·g^-1, respectively. Conclusion The established HPLC characteristic fingerprint and content determination method are accurate and reliable. chlorogenic acid, vicenin-2, and isovitexin can be used as Q-Markers for the anti-hyperuricemia effect of U. cannabina, providing a basis for the quality evaluation and control of the medicinal material.

R285.5；R291.4

国家重点研发计划资助项目(2019YFC1712300); 内蒙古自然科学基金博士项目(2020BS08006); 内蒙古民族大学博士科研启动基金项目(BS629);内蒙古民族大学蒙医药研发教育部重点实验室开放课题项目(MDK2023075,MDK2024044);内蒙古民族大学国家药品监督管理局中药(蒙药)质量控制实验室开放课题(MDK2025025)