目的 通过游泳劳损联合放血法诱导的气血亏虚大鼠模型，大黄酸诱导钠钾ATP酶（Na⁺，K⁺-ATPase）抑制的炎性HCT-116细胞模型，分别考察乌鸡白凤软胶囊（WJBF）补气养血及固涩作用。方法 将雌性SD大鼠随机分为对照组、模型组、复方阿胶浆（EJJ，6.2 mL·kg_-1）组、定坤丹（1.4 g·kg_-1）组和WJBF低、中、高剂量（0.3、0.6、1.2 g·kg_-1）组，每组10只，ig给药，每天给药1 h后，除对照组外其余各组大鼠进行力竭游泳，记录力竭游泳时间，持续21 d，于第1、8、15天按照5 mL·kg_-1眼眶放血。第21天给药1 h后，观察大鼠一般状态并进行表观评分，评分结束后开始力竭游泳实验并记录时间；第22天给药1 h后，腹主动脉取血，血液细胞分析仪检测各组大鼠全血白细胞（WBC）、红细胞（RBC）、血红蛋白（HGB）、血小板（PLT）水平以及红细胞比容（HCT）、网织红细胞（RET）百分比的变化，并通过试剂盒法测定血浆免疫造血相关指标［促血小板生成素（TPO）、巨噬细胞集落刺激因子（GM-CSF）、促红细胞生成素（EPO）］、脾脏与胸腺指数、肝糖原、肌糖原水平。体外培养HCT-116细胞，在有、无大黄酸50 μmol·L-1诱导2种情况下，添加WJBF低、中、高浓度（200、400、800 μg·mL^-1）作用24 h，通过试剂盒法检测细胞裂解液中Na⁺，K⁺-ATPase活性。结果 与模型组相比，WJBF中、高剂量组表观评分均显著降低（P＜0.05），游泳时间均显著延长（P＜0.01、0.001）；低、中、高剂量组RBC（P＜0.05、0.01）和PLT（P＜0.05、0.01、0.001）显著上升，高剂量组RET显著增加（P＜0.05），中剂量组HGB显著增加（P＜0.05）；中、高剂量组的肝糖原（P＜0.001）和肌糖原（P＜0.01）都显著升高，低剂量组肌糖原显著升高（P＜0.001），高剂量组血浆中EPO表达量显著降低（P＜0.05），中剂量组TPO表达量显著升高（P＜0.001）；低、中剂量组均具有升高GM-CSF表达量的趋势（P＞0.05）。在细胞正常状态和炎性状态下，WJBF高剂量对细胞膜Na⁺，K⁺-ATPase的活性均具有显著增强的作用（P＜0.05、0.01）。结论 WJBF对游泳劳损联合放血诱导的气血亏虚大鼠具有较好的治疗效果；WJBF可以通过增强细胞膜Na⁺，K⁺-ATPase活性发挥固涩的作用。

Objective To investigate the therapeutic effects of Wuji Baifeng Soft Capsule (WJBF) on replenishing qi, nourishing blood, and inducing astringency, we established a rat model of qi and blood deficiency induced by exhaustive swimming combined with bloodletting, along with an inflammatory HCT-116 cell model in which sodium-potassium-ATPase (Na⁺/K⁺-ATPase) activity was inhibited by rhein. Methods Female SD rats were randomly divided into the control group, model group, Ejiao Compound Syrup (EJJ, 6.2 mL·kg^-1) group, Dingkun Dan (DKD, 1.4 g·kg^-1) group and low-, medium- and high-dose WJBF (0.3, 0.6, 1.2 g·kg^-1) groups, with 10 rats in each group. The rats were administered ig and 1 h after administration, except for the control group, the rats in the other groups were subjected to exhaustive swimming. The exhaustive swimming time was recorded and the treatment lasted for 21 d. On the 1st, 8th and 15th d, blood was collected from the orbital vein at a dose of 5 mL·kg^-1. On the 21st d, 1 h after administration, the general condition of the rats was observed and scored. After the scoring, the exhaustive swimming experiment was conducted and the time was recorded. On the 22nd d, 1 h after administration, blood was collected from the abdominal aorta. The levels of white blood cells (WBC), red blood cells (RBC), hemoglobin (HGB), platelets (PLT), hematocrit (HCT), and reticulocytes (RET) percentage in whole blood were detected by a blood cell analyzer. The levels of plasma immune hematopoietic-related indicators [thrombopoietin (TPO), granulocyte-macrophage colony-stimulating factor (GM-CSF), erythropoietin (EPO)], spleen and thymus indices, liver glycogen, and muscle glycogen were determined by kit methods. HCT-116 cells were cultured in vitro. In the presence or absence of 50 μmol·L^-1 rhein induction, WJBF at low, medium and high concentrations (200, 400, 800 μg·mL^-1) were added and the cells were incubated for 24 h. The activity of Na⁺, K⁺-ATPase in the cell lysate was detected by kit methods. Results Compared with the model group, the apparent scores of the medium and high-dose WJBF groups were significantly reduced (P < 0.05), and the swimming time was significantly prolonged (P < 0.01, 0.001). The RBC (P < 0.05, 0.01) and PLT (P < 0.05, 0.01, 0.001) in the low, medium and highdose groups were significantly increased, and the RET in the high-dose group was significantly increased (P < 0.05), and the HGB in the medium-dose group was significantly increased (P < 0.05). The liver glycogen (P < 0.001) and muscle glycogen (P < 0.01) in the medium and high-dose groups were significantly increased, and the muscle glycogen in the low-dose group was significantly increased (P < 0.001). The expression of EPO in the plasma of the high-dose group was significantly decreased (P < 0.05), and the expression of TPO in the medium-dose group was significantly increased (P < 0.001). The low and medium-dose groups both showed a trend of increasing GM-CSF expression (P > 0.05). Under normal and inflammatory conditions of cells, the high-dose WJBF had a significant enhancing effect on the activity of Na⁺, K⁺-ATPase in the cell membrane (P < 0.05, 0.01). Conclusion WJBF exerts notable therapeutic effects in a rat model of qi and blood deficiency induced by swimming fatigue and bloodletting. Its astringent effect is likely mediated through enhancement of cell membrane Na⁺/K⁺-ATPase activity.

R285.5

国家科技重大专项—“四大慢病重大专项”（2025ZD0549700）