目的 观察左金丸对葡聚糖硫酸钠（DSS）诱导溃疡性结肠炎小鼠巨噬细胞极化和Nogo-B/RhoA信号通路的影响。方法 将40只C57BL/6J雄性小鼠随机分为对照组、模型组、左金丸(0.3 g·kg^-1)组及美沙拉嗪肠溶片(5-ASA,300 mg·kg^-1)组，每组10只。除对照组外，其余各组采用“2.3% DSS（第1～7天）—自由饮水（第8～14天）—2.3% DSS（第15～21天）”制备小鼠实验性结肠炎模型。造模第8天开始ig给药，持续14 d。每天定时监测小鼠体质量，计算每日疾病活动指数（DAI）；造模第22天，观察小鼠一般情况和结肠病理变化；采用酶联免疫吸附试验（ELISA）检测小鼠结肠组织肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、转化生长因子-β1（TGF-β1）和白细胞介素-10（IL-10）含量；采用流式细胞术检测结肠组织CD11b⁺F4/80⁺细胞中MHC-II⁺、CD64⁺、CD206⁺、CD209⁺表达水平；采用Western blotting法测定结肠组织内质网膜蛋白4B（Nogo-B）、Rho关联含卷曲螺旋结合蛋白激酶1（Rock1）和Ras同源家族成员A（RhoA）蛋白表达水平；采用实时荧光定量PCR（q RT-PCR）法测定结肠组织Nogo-B和RhoA mRNA表达水平。结果 与模型组比较，左金丸显著改善DSS诱导结肠炎小鼠体质量降低、DAI升高、结肠长度降低、结肠质量降低、结肠指数升高、病理损伤评分升高（P<0.05、0.01）；降低结肠组织TNF-α、IL-1β、CD11b⁺F4/80⁺MHC-II⁺、CD11b⁺F4/80⁺CD64⁺细胞水平，以及Nogo-B、RhoA、Rock1蛋白和mRNA的表达（P<0.05、0.01）；同时提高IL-10、TGF-β1、CD11b⁺F4/80⁺CD206⁺、CD11b⁺F4/80⁺CD209⁺细胞水平（P<0.05、0.01）。结论 左金丸对DSS诱导小鼠结肠炎具有明显缓解作用，其作用机制可能与抑制Nogo-B/RhoA信号通路，调控巨噬细胞M1/M2极化，进而恢复促炎/抗炎因子平衡相关。

Objective To observe the effect of Zuojin Pill on dextran sodium sulfate（DSS）-induced macrophage polarization and Nogo-B/Rho A signaling pathway in ulcerative colitis mice. Methods Forty C57BL/6J male mice were randomly divided into control group, model group, Zuojin Pill(0.3 g·kg^-1) group, and 5-Aminosalicylic Acid Enteric Tables(5-ASA, 300 mg·kg^-1) group. Except for the control group, the rest of the groups were treated with "2.3% DSS（1st to 7th d）—free drinking water（8th to 14th d）—2.3% DSS（15th-21st d）" to replicate experimental colitis in mice. Ig administration began on day 8 of modeling and lasted for 14 d. On the 22nd d of modeling, the general condition and pathological changes of the colon of mice were observed. The expression levels of tumor necrosis factor（TNF-α）, interleukin-1β（IL-1β）, transforming growth factor-β1（TGF-β1） and interleukin-10（IL-10） were detected by enzyme-linked immunosorbent assay. The expression levels of MHC-II⁺, CD64⁺, CD206⁺ and CD209⁺ in CD11b⁺F4/80⁺ cells in colon tissue were detected by flow cytometry. Western blotting was used to determine reticulon protein family 4B（Nogo-B）, recombinant Rho associated coiled coil containing protein kinase 1（Rock1）, and Ras homolog family member A（Rho A） protein expression level; Reverse transcription-polymerase chain reaction（q RT-PCR） was used to determine the mRNA expression levels of Nogo-B and RhoA in colon tissue. Results Compared with model group, Zuojin Pill significantly improved the body weight change rate, disease activity index, colon length, colon mass, intestinal weight index, pathological injury score and pathological morphology of DSS-induced colitis mice（P ＜ 0.05 and 0.01）; Reduced the levels of TNF-α, IL-1β, CD11b⁺F4/80⁺MHC-II⁺, CD11b⁺F4/80⁺CD64⁺, and the expression of Nogo-B, Rho A, Rock1 protein and mRNA in colon tissues. At the same time, the levels of IL-10, TGF-β1, CD11b⁺F4/80⁺CD206⁺ and CD11b⁺F4/80⁺CD209⁺ were up-regulated（P ＜ 0.05 and 0.01）. Conclusion Zuojin Pill has a significant alleviating effect on DSSinduced colitis in mice, and its mechanism of action may be to regulate macrophage M1/M2 polarization by inhibiting the NogoB/Rho A signaling pathway, thereby restoring the balance of pro-inflammatory/anti-inflammatory factors.

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国家自然科学基金资助项目(82160870); 江西省教育厅科技计划项目(GJJ2200910); 江西省中医药中青年骨干人才项目培养计划(赣中医药科教字[2022]7号); 江西中医药大学省级大学生创新创业训练计划(S202510412126);江西中医药大学校级研究生创新专项基金(XJ-S202456)