目的 采用超高效液相色谱-四极杆静电场轨道阱质谱（UPLC-Orbitrap-MS/MS）技术对巴戟天化学成分快速分析，并开展环烯醚萜类成分抗类风湿性关节炎（RA）的网络药理学研究。方法 运用UPLC-Orbitrap-MS/MS技术在正、负离子模式下分别对巴戟天中化学成分进行定性分析，利用UPLC-MS/MS技术、proteowizard和XCMS软件、辅助应用公共数据库和自建中药物质库，表征巴戟天化学成分。利用网络靶点数据库获取已鉴定到的环烯醚萜类成分的作用靶点和RA相关疾病靶点信息；采用DAVID数据库对核心靶点进行基因本体（GO）和京都基因与基因组百科全书（KEGG）富集分析；借助GeneMANIA平台绘制交集蛋白质-蛋白质相互作用（PPI）网络筛选关键靶点；利用分子对接技术聚焦环烯醚萜类成分作用靶标。结果 利用UPLC-Orbitrap-MS/MS技术共鉴定出巴戟天中的370个化学成分，共检测出环烯醚萜类物质4个；筛选了4个环烯醚萜类物质的药效作用靶点318个，RA疾病靶点1 001个，交集靶点69个。GO 478条和KEGG富集到相关通路137条，最终确定环烯醚萜类成分可能通过肿瘤坏死因子α（TNF-α）、Janus激酶2（JAK2）、信号传导及转录激活蛋白3（SATA3）和蛋白酪氨酸磷酸酶受体C（PTPRC）关键靶点，协同调控TNF-α、磷脂酰肌醇3激酶（PI3K）-苏氨酸激酶（Akt）和JAK-STAT等信号通路发挥抗RA的治疗作用。分子对接得分显示水晶兰苷与关键靶点较好地结合。结论 巴戟天中的环烯醚萜类成分可能通过干预TNF、JAK2、SATA3和PTPRC靶点，调控PI3K-Akt和JAK-STAT等信号通路发挥抗RA的治疗作用。

Objective To rapidly analyze the chemical constituents of Morinda officinalis using ultra-performance liquid chromatography coupled with quadrupole-orbitrap tandem mass spectrometry (UPLC-Orbitrap-MS/MS) and to investigate the antirheumatoid arthritis (RA) effects of its iridoid glycosides through network pharmacology.Methods Qualitative analysis of the chemical constituents in M. officinalis was conducted using UPLC-Orbitrap-MS/MS technology in both positive and negative ion modes. The characterization of chemical components was achieved through the application of UPLC-MS/MS technology, complemented by ProteoWizard and XCMS software platforms, with additional support from public databases and a proprietary traditional Chinese medicine substance library. Network target databases were systematically utilized to identify the target proteins of the detected iridoid components and the disease targets associated with RA. The DAVID database was employed for Gene Ontology (GO) functional annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the core targets. The GeneMANIA platform was used to construct a protein-protein interaction (PPI) network of overlapping targets to screen key targets. Molecular docking technology was applied to focus on monotropein.Results A total of 370 chemical constituents were identified in M. officinalis using UPLC-Orbitrap-MS/MS technology, including four iridoid glycosides. Through systematic screening, 318 potential therapeutic targets of these iridoid glycosides and 1 001 RA-related targets were identified, with 69 overlapping targets identified. Functional enrichment analysis revealed 478 significant GO terms and 137 KEGG pathways. The results suggest that iridoid glycosides may exert anti-RA therapeutic effects through key targets including TNF, JAK2, STAT3, and PTPRC, potentially modulating critical signaling pathways such as tumor necrosis factor, PI3K-Akt, and JAK-STAT. Molecular docking analysis indicated that monotropein exhibited superior binding affinity compared to other constituents.Conclusion Iridoid glycosides of M. officinalis may exert anti-RA therapeutic effects by targeting TNF, JAK2, STAT3, and PTPRC, and by regulating signaling pathways such as PI3KAkt and JAK-STAT to promote FLS apoptosis.

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国家自然科学基金青年科学基金项目（82104733）；海南省“南海新星”科技创新人才平台项目（NHXXRCXM202317）；孙申田青年人才基金（2021SC-01）；海南省自然科学基金（825QN330）