目的 考察注射用丹参多酚酸（SAFI）对大脑中动脉缺血再灌注（MCAO/R）大鼠脑水肿的药效作用。方法 线拴法构建MCAO/R大鼠模型，将造模成功的大鼠随机分为模型组、布美他尼（阳性药，0.2 mg·kg⁻¹）组和SAFI低、中、高剂量（5.80、11.61、23.31 mg·kg⁻¹）组，假手术组大鼠仅做血管分离，不插入线栓。每天1次、连续尾iv给药14 d，假手术组和模型组给予0.9%氯化钠注射液。给药前后对各组大鼠进行称质量和神经功能评分；给药后采用TTC染色法检测脑梗死体积，称质量法检测脑含水量，核磁检测脑水肿体积、水分子表观弥散系数（ADC）；HE染色观察脑组织病理形态。结果 给药前大鼠体质量及神经功能评分无差异，给药14 d后，与模型组比较，SAFI低、中、高剂量组和布美他尼组体质量明显增加（P＜0.01、0.001），神经功能评分显著降低（P＜0.01、0.001），脑组织梗死体积显著缩小（P＜0.001），脑含水量显著降低（P＜0.01、0.001），核磁T2加权成像（T2WI）序列显示脑水肿体积显著减少（P＜0.01、0.001），弥散加权成像（DWI）序列显示ADC值显著升高（P＜0.05、0.001）；HE染色结果显示SAFI对神经细胞肿大和空泡变性均有改善作用。结论 SAFI对MCAO/R大鼠脑水肿具有明显改善作用。

Objective To investigate the effect and mechanism of Salvianolic Acids for Injection (SAFI) on cerebral edema in MCAO/R rats. Methods The rat model of middle cerebral artery ischemia-reperfusion (MCAO/R) was established by suture method. The successfully modeled rats were randomly divided into model group, SAFI low, medium, and high dose (5.80, 11.61, and 23.21 mg·kg⁻¹) group, and bumetanide (positive drug, 0.2 mg·kg⁻¹) group, with 20 rats in each group. The sham operation group and the model group were given 0.9 % sodium chloride injection once a day for 14 days. The body weight and neurological function score of rats in each group were measured before and after administration. After administration, the volume of cerebral infarction was detected by TTC staining. Brain water content was detected after administration, and nuclear magnetic detection was performed before and after administration. HE staining was used to observe the pathological morphology of brain tissue. Results There was no difference in body weight and neurological function score of rats before administration. After 14 days of administration, compared with the model group, the body weight of SAFI low middle and high dose (5.80, 11.61, and 23.21 mg·kg⁻¹) group, and bumetanide group increased significantly (P < 0.01 and 0.001), the neurological function score decreased significantly (P < 0.01 and 0.001), the infarct volume of brain tissue decreased significantly (P < 0.001), and the brain water content was significantly lower than that of the model group (P< 0.05 and 0.001). Magnetic resonance T2 weighted imaging (T2WI) sequence showed a significant decrease in brain edema volume (P < 0.01, 0.001), while diffusion-weighted imaging (DWI) sequence showed a significant increase in ADC value (P < 0.05, 0.001). HE staining results showed that SAFI could improve the swelling and vacuolar degeneration of nerve cells. Conclusion SAFI has a significant improvement effect on cerebral edema in MCAO rats.

R285.5

天津市制造业高质量发展专项资金—天津天士力之骄药业有限公司技术中心创新能力建设（ZZY20232088）