目的 探讨丁香花蕾乙醇提取物（AEC）在 39 ℃条件下对口腔鳞状细胞癌增殖、迁移、凋亡的影响。方法 设置空白对照组、溶剂对照组和0.05%、0.10%、0.20% AEC组，分别于39 ℃条件下作用于口腔鳞状细胞癌细胞培养72 h。采用CCK-8和克隆形成法检测口腔鳞状细胞癌的增殖能力；划痕实验检测细胞的迁移能力；流式细胞术和 Hoechest 33342染色检测细胞的凋亡。结果 与对照组和模型组比较，0.05%、0.10%、0.20%组口腔鳞状细胞增殖活性逐渐降低，细胞克隆形成随着浓度增加集落形成降低，细胞迁移能力和侵袭能力逐渐减弱；细胞凋亡率逐渐增加，均与 AEC 呈浓度相关性。在39 ℃条件下，口腔鳞状细胞癌增殖、迁移和侵袭的抑制作用更加明显，细胞凋亡更加显著。结论 AEC对口腔鳞状细胞癌的增殖、迁移具有抑制作用，且能促进口腔鳞状细胞癌凋亡，在39 ℃条件下，具有协同作用，对口腔鳞状细胞癌增殖、迁移、凋亡的作用更明显。

Objective To investigate the effects of alcohol extract of clove (AEC) combined with 39 ℃ on the proliferation, migration and apoptosis of oral squamous cell carcinoma (OSCC). Methods Oral squamous cells (OSC) were cultured with 0, 0.05%, 0.10%, or 0.20% AEC at 39 ℃ for 72 h. CCK-8 and colony formation assays were used to detect the proliferation of OSCC. The migration ability of cells was detected by scratch test. The apoptosis of the cells was detected by flow cytometry and Hoechest 33342 staining. Results Compared with the control and model group, the 0.05%, 0.10% and 0.20% groups showed a gradual decrease in the proliferation activity of OSC. With the increase of the concentration of 0.05%, 0.10%, and 0.20% groups, the colony formation decreased, and the cell migration and invasion ability gradually decreased. The apoptosis rate of OSC increased gradually, both in a concentration dependent manner with AEC. Combined with 39 ℃ treatment, the inhibition of proliferation, migration and invasion of OSC was more obvious, and cell apoptosis was more significant. Conclusion AEC can inhibit the proliferation and migration of OSCC, and promote the apoptosis of OSCC. When used in combination with 39 ℃, it has a synergistic effect, so as to achieve the therapeutic effect of OSCC.

R285.5

国家自然科学基金资助项目（82060489）；贵州省科技技术厅资助项目（黔科合基础-ZK［2024］一般 404）