间充质干细胞来源外泌体(MSCs-Exo)近年来成为替代间充质干细胞发挥治疗作用的研究热点,良好的外泌体分离和贮存方法是保障获得的外泌体具有稳定的质量和可重复的关键,也是临床转化的前提。外泌体具有多种分离方式,包括国际金标准的超速离心法,改良后的蔗糖密度梯度离心法、超滤法以及微流控技术等。贮存温度和贮存介质是影响外泌体生物学完整性的主要因素。结合当下MSCs-Exo的研究现状及其分离和贮存条件的研究进展进行综述,以期为临床转化研究提供参考。

Mesenchymal stem cell derived exosomes (MSCs-Exo) has become a research hotpot in the field of replace Mesenchymal Stem Cell (MSCs) on the therapeutic recently. A good method of exosome separation and storage is the key to ensure the stable quality and repeatability of the obtained exosomes, and is also the premise of clinical transformation. Exosomes have a variety of separation methods, including international gold standard ultrafiltration method, improved sucrose density gradient centrifugation method, ultrafiltration method and microfluidic technology. Storage temperature and storage medium are the main factors affecting the biological integrity of exosomes. This article reviews the current research status of MSCs-Exo and their isolation and storage conditions in order to provide reference for translational research.

R965.3

中国医学科学院医学与健康科技创新工程项目(2019-I2M-5-020);天津市科技计划项目:细胞产品开发和临床应用的研究(22ZYJDSY00150);天津市科技计划项目:细胞制品的成药性及转化研究(23ZGCXQY00050)