目的 鉴定当归-川芎药对化学成分及大鼠ig后的入血原型成分和代谢产物。方法 基于超高效液相色谱-静电场轨道阱-高分辨率质谱技术（UPLC-Orbitrap-HRMS），结合对照品图谱、自建数据库、相关文献信息及Compound Discoverer3.3、Xcalibur qual browser 4.3等软件对当归-川芎药对的化学成分进行分析鉴定；大鼠经ig给药后制得含药血清，通过比对含药血清与对照组血清，鉴定入血原型成分和代谢产物。结果 共在当归-川芎药对中鉴定出69个化学成分，主要包括苯酞类24个、有机酸类24个、氨基酸类8个、含氮类7个、香豆素类4个、木脂素类1个、黄酮类1个；在含药血清中共鉴定出30个入血原型成分，包括苯酞类15个、有机酚酸类13个、香豆素类2个；鉴定出45个代谢产物，包括32个苯酞类代谢产物，13个有机酚酸类代谢产物。结论 明确了当归-川芎药对的化学成分及入血原型成分和代谢产物。

Objective To identify the chemical components of Angelicae Sinensis Radix-Chuanxiong Rhizoma herb pairs and their prototype components into blood and metabolites after oral administration in rats. Methods Based on the ultra-performance liquid chromatography coupled with Orbitrap high-resolution mass spectrometry technology (UPLC-Orbitrap-HRMS), the chemical components of Angelicae Sinensis Radix-Chuanxiong Rhizoma herb pairs were analyzed and identified in combination with reference substance, self-established database, relevant literature information, Compound Discoverer 3.3, and Xcalibur qual browser 4.3 software; Drug-containing serum were prepared from normal rats by intragastric administration. The prototype components and metabolites were identified by comparing drug-containing serum with blank serum. Results A total of 69 chemical components were identified in the Angelicae Sinensis Radix-Chuanxiong Rhizoma herb pairs, including 24 phthalides, 24 organic acids, eight amino acids, seven nitrogen-containings, four coumarins, one lignan and one flavonoid. A total of 30 prototype components were identified in the drug-containing serum, including 15 phthalides, 13 organic acids, and two coumarins; 45 metabolites were identified in vivo, including 32 phthalides and 13 organic acids. Conclusion The chemical components, prototype components, and metabolites of Angelicae Sinensis Radix-Chuanxiong Rhizoma herb pairs were clarified.

R284.1

安徽省高校自然科学研究项目（KJ2021A0595）