目的 考察生姜不同时机给予正常小鼠后对其产热能力的影响。方法 将C57BL/6小鼠随机分为早上对照组、早上给姜组、晚上对照组和晚上给姜组,并分别于早、晚ig生姜水提液(以生姜生药量计为3.25 g·kg^-1或0.9%氯化钠溶液,连续ig 2周。测量小鼠体质量及体温变化,并利用冷暴露实验测试小鼠抗寒能力。分别于早、晚末次给姜后1 h取材,计算脂肪脏器指数,HE染色观察脂肪组织病理变化,实时荧光定量PCR(qRT-PCR)法检测棕色脂肪和腹股沟皮下白色脂肪(iWAT)中产热基因线粒体棕色脂肪解偶联蛋白1(Ucp1)和时钟基因(Bmal1、Per2)表达情况。结果 早上对照组和晚上对照组小鼠体质量、体温、抗寒能力、脂肪形态及产热基因各项指标均无显著性差异。与晚上对照组相比,晚上给姜组小鼠体质量有减轻的趋势、给姜后体温升高(P＜0.05)、抗寒能力显著增强(P＜0.05),并显著减小脂肪细胞形态,同时显著增加脂肪细胞中Ucp1的表达(P＜0.05),但对脂肪组织Bmal1、Per2表达无显著影响。与早上对照组相比,早上给姜组小鼠体质量有增加的趋势,给姜后对基础体温、抗寒能力、脂肪组织Ucp1表达无显著影响,但显著降低脂肪组织中Bmal1的表达、升高Per2的表达(P＜0.05)。结论 早、晚不同时机给予生姜对正常小鼠产热能力具有差异化的影响,其中晚上ig生姜水提液可以显著增强小鼠产热能力,其机制可能与生姜影响生物钟基因表达和促进棕色脂肪产热、白色脂肪棕色化有关。

Objective This study uses ginger as a tool drug to investigate the effects of different administration times on the thermogenesis in normal mice.Methods C57BL/6 mice were randomly divided into normal control group in the morning(MC),ginger gavage group in the morning(MG), normal control group in the evening(EC), and ginger gavage group in the evening(EG),and were given ginger water extract or normal saline in the morning or evening respectively for two weeks. The body weight and body temperature of mice were measured, and the cold exposure test was used to test the cold tolerance of mice. The mice were sacrificed at one hour after the last administration of ginger in the morning or evening, and the fat organ index was calculated. The pathological changes of adipose tissue were observed by HE staining. Real time fluorescence quantitative PCR(qRT-PCR) was used to detect the expression of mitochondrial brown fat uncoupling protein 1(Ucp1) and clock genes(Bmal1, Per2) in brown adipose tissue and subcutaneous white adipose tissue(iWAT) of the groin.Results There were no significant differences in body weight,body temperature, cold resistance, fat morphology and thermogenic genes between MC and MG group. Compared with EC group,the mice in EG group showed a trend of weight loss, a continuous increase in body temperature(P＜0.05), a significant increase in cold resistance(P＜0.05), a significant reduction in the morphology of adipocytes, and a significant increase in the expression of thermogenic gene(Ucp1) in adipocytes(P＜0.05). However, it had no effect on the expression of core clock genes(Bmal1, Per2) in adipose tissue. Compared with MC group, the MG group showed an increasing trend in body weight. It had no effect on basal body temperature, cold resistance, adipose tissue morphology and expression of thermogenic gene(Ucp1), but significantly affected the expression of Bmal1, and Per2 in adipose tissue(P＜0.05).Conclusion This study demonstrates for the first time that ginger administration at different times, in the morning or evening, has differential effects on thermogenesis in mice, and ginger administration in the evening can significantly enhance thermogenesis in mice. The mechanism may be related to the effect of ginger on the expression of circadian clock genes and the promotion of thermogenesis in brown adipose tissue and the browning of white adipose tissue.

R285.5

糖尿病认知损伤脑单细胞转录图谱及滋肾方防治配伍机制研究(22HHZYSS00015)