Notch signaling pathway evolved conservatively. The Notch signaling pathways include 'canonical' and 'non-canonical' pathways. Physiologically, the intracellular domain NICD is released into the nucleus after Notch receptor is cleaved three times, and then regulates biological processes such as animal development, tissue renewal and repairment by regulating the transcription of target genes in nucleus. Notch genetic mutations or signaling abnormalities can promote or inhibit the progression of various diseases and the mechanism is complex in cancer and non-cancer diseases. A series of drugs candidates involved in the Notch signaling pathway have been evaluated although there are no drugs on the market yet. Therefore, it is necessary to study the Notch pathway thoroughly.