目的 研究跌打七厘片对小鼠骨折愈合的影响。方法 将100只C57BL/6小鼠随机分为空白对照组20只、假手术组20只、造模组60只。造模组制备股骨骨折模型，造模后再随机分为模型组和跌打七厘片低、高剂量（405.5、1 621.8 mg·kg^-1）组，每组20只。每天1次，连续ig给药28 d。每周通过骨组织形态学和苏木精伊红（HE）染色观察骨痂形态改变、微计算机断层扫描分析（Micro-CT）检测愈合骨的骨体积分数（BV/TV），生物力学三点弯曲实验检测愈合骨的最大负载，全自动生化仪检测小鼠血清钙（Ca）、磷（P）、碱性磷酸酶（ALP）的水平。结果 HE染色、Micro-CT结果显示，造模后第14天，与模型组比较，跌打七厘片组有较多的软骨细胞浸润，钙化编织骨骨量多，新生骨痂骨量明显较多；造模后第28天，模型组骨折断端仍有未连接，而跌打七厘片组的骨折断端均已连接，钙化编织骨转化为层状骨，逐渐恢复骨的基本形态，有更多的新生骨痂及钙化程度，高剂量组骨折断端被钙化新生骨完全填充并与周围骨皮质相联合。造模后第14天，跌打七厘片高剂量组的BV/TV、最大负载显著高于模型组（P<0.05）；造模后第28天，低、高剂量组的BV/TV、最大负载均显著高于模型组（P<0.05）。造模后小鼠血清Ca水平各组之间没有明显差异。造模后小鼠血清P、ALP水平呈上升趋势，并在21 d达到高峰，与模型组比较，第14、21天跌打七厘片低剂量组和第7、14、21、28天的高剂量组血清P水平显著升高（P<0.05），第14、21、28天的高剂量组血清ALP水平显著升高（P<0.05）。结论 跌打七厘片可以提高成骨活性及钙盐沉积，以增加骨痂水平增强骨强度，有利于小鼠骨折愈合。

Objective To study the effect of Dieda Qili Tablet on fracture healing in mice. Methods A total of 100 C57BL/6 mice were randomly divided into blank control group (n=20), sham operation group (n=20) and model group (n=60). After modeling, the model group was randomly divided into model group, Dieda Qili Tablets low and high-dose (405.5 and 1 621.8 mg·kg^-1) group, with 20 rats in each group. Once a day, continuous ig administration for 28 days. Micro-CT three-dimensional imaging analysis was used to detect the bone volume fraction of the healed bone. Biomechanical three-point bending test was used to detect the maximum load of the healed bone. Serum Ca, P, and alkaline phosphatase (ALP) contents were detected by fully automatic biochemical analyzer. Results HE staining and Micro-CT results showed that 14 days after modeling, compared with the model group, the Dieda Qili Tablets group had more chondrocyte infiltration, more calcified woven bone mass, and significantly more new callus bone mass. Totally 28 days after modeling, the fracture ends of the model group were still unconnected, while the fracture ends of the drop seven millimeter piece group were all connected. The calcified woven bone transformed into layered bone, gradually restoring the basic form of the bone, with more new callus and degree of calcification. The fracture ends of the high-dose group were completely filled with calcified new bone and combined with the surrounding bone cortex. On the 14th day after modeling, the BV/TV and maximum load of the high-dose group of Dieda Qili Tablets were significantly higher than those of the model group (P < 0.05). On the 28th day after modeling, the BV/TV and maximum load of the low and high dose groups were significantly higher than those of the model group (P < 0.05). After modeling, there was no significant difference in serum Ca levels among the groups. After modeling, the serum P and ALP levels of the model mice showed an upward trend and reached their peak on the 21st day. Compared with the model group, the serum P levels in the low-dose group of DiDa Qili Tablet on the 14th and 21st days and the high-dose group on the 7th, 14th, 21st, and 28th days were significantly increased (P < 0.05), and the serum ALP levels in the low and highdose group significantly increased on days 14, 21, and 28 (P < 0.05). Conclusion Dieda Qili Tablet has therapeutic effect on fracture in mice by improving osteogenic activity and calcium salt deposition to increase the content of callus and enhance bone strength.

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上海市中医药“三年行动计划”［ZY（2021-2023）-0211］；上海市“科技创新行动计划”生物医药科技支撑专项（20S21902100）；上海市教委协同创新中心：中西医结合-中成药临床评价平台（A1-U21-205-0103）；上海市申康中心示范性研究型病房建设（SHDC2022CRW010）；上海市申康中心医企融合创新协同专项（SHDC2022CRT018）；上海市慢性筋骨病临床医学研究中心项目（20MC1920600）