目的 基于HPLC指纹图谱、多成分定量结合化学模式识别法评价黔钩藤质量。方法 从贵州中药材市场随机购买了18批钩藤饮片，采用HPLC法建立了18批钩藤饮片的指纹图谱；通过中药色谱指纹图谱相似度评价系统（2012年版）进行相似度评价，对其中5种生物碱进行了指认，并采用外标法和一测多评法进行了含量测定；采用SPSS 26.0统计软件进行聚类分析和主成分分析。结果 在钩藤饮片指纹图谱中，确定了共有峰28个，采用对照品指认了5个生物碱成分，分别为异去氢钩藤碱、异钩藤碱、去氢钩藤碱、钩藤碱和去氢毛钩藤碱，18批钩藤的相似度评价表明市场上钩藤的质量差异较大；聚类分析将18批钩藤饮片样品聚为3大类；主成分分析用5个主成分进行综合评价，其中8批钩藤饮片的综合得分大于1；采用一测多评法和外标法计算18批钩藤饮片中5种生物碱的含量，经GraphPad差异性分析表明，2种方法无显著性差异。结论 市场上钩藤质量差异较大，建立了检测5个生物碱成分的一测多评法和外标法，为完善钩藤质量评价提供参考。

Objective To evaluate the quality of Uncaria rhynchophylla collected in Guizhou province based on combinative methods of HPLC fingerprint and multi-components determination and chemical pattern recognition. Methods Tottally 18 batches of U. rhynchophylla were randomly purchased from Guizhou traditional Chinese medicine market. The fingerprint of 18 batches of U. rhynchophylla was established by HPLC. The similarity of 18 batches was analyzed with Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia (2012). Five alkaloids were identified, and their contents were determined by external standard method and one test and multiple evaluations (QAMS). The hierarchical clustering analysis (HCA) and principal component analysis (PCA) were performed by SPSS 26.0. Results Totally 28 peaks were identified in the fingerprint of U. rhynchophylla and five of the common peaks were identified as isocorynoxeine, isorhynchophylline, corynoxeine, rhyncholphylline, and hirsuteine by reference substance. The fingerprint similarities of the 18 batches indicate that the quality of U. rhynchophylla in the market varied greatly. According to HCA, the samples were divided into three groups. Five principal components were selected for comprehensive evaluation by PCA, and the comprehensive scores of eight batches were greater than one. The contents of five alkaloids in 18 batches were calculated by the methods of external standard method and QAMS. GraphPad was used for difference analysis, and the results showed that there was no significant difference between the two methods. Conclusion This study showed that the quality of U. rhynchophylla differed greatly in the market, we have successfully established the multiple evaluation method and the external standard method for the determination of five alkaloid components, providing a reference for the improvement of the quality evaluation of uncaria hook.

R284.1

贵州省科技计划项目［黔科合基础-ZK（2021）540］；贵州省科技计划项目［黔科合基础-ZK（2021）535］；贵州中医药大学博士启动基金［3043-043190010］