目的 探讨注射用益气复脉（冻干）（YQFM）对失血性休克大鼠的药效作用。方法 通过股动脉放血的方法建立失血性休克大鼠模型，随机将造模成功的大鼠分为模型组、肾上腺素（10 μg·kg^-1盐酸肾上腺素注射液）组、YQFM低和高剂量（232.2、464.3 mg·kg^-1，464.3 mg·kg^-1为临床等效剂量）组、联合给药（肾上腺素10 μg·kg^-1＋YQFM 464.3 mg·kg^-1）组，每组10只，假手术组只切口不放血。给药结束后，观察给药3 h内大鼠存活情况；使用八通道无创血压仪监测造模前、造模后、给药3 h后的收缩压；酶联免疫（ELISA）法检测各组大鼠血清中肌酸激酶同工酶（CK-MB）、乳酸脱氢酶（LDH）、丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）和超氧化物歧化酶（SOD）水平。结果 造模后大鼠的平均动脉压均维持在30～40 mm Hg，表明造模成功。假手术组及各给药组大鼠在3 h内全部存活，模型组死亡3只，存活率为70%，平均生存时间为（166.00±23.66） min。各组大鼠的基础血压无显著性差异，模型组和各给药组大鼠造模后收缩压显著降低，与假手术组比较差异显著（P<0.001）；与模型组比较，给药3 h后各给药组收缩压均显著升高（P<0.001），联合给药组升压效果最好。与模型组相比，肾上腺素组血清CK-MB、LDH、ALT、AST水平均显著下降（P<0.01）； YQFM低剂量组LDH、ALT、AST水平显著降低（P<0.05）； YQFM高剂量组与联合给药组血清CK-MB、LDH、ALT、AST水平显著降低，SOD水平显著升高（P<0.05、0.01、0.001），联合给药对血清生化指标的改善作用最好。结论 YQFM可以明显提高失血性休克大鼠的收缩压水平，改善血清中相关生化指标水平，且与肾上腺素联用具有一定的协同作用。

Objective To investigate the effect of Yiqi Fumai Lyophilized Injection (YQFM) on the pharmacodynamics of hemorrhagic shock rats. Methods The hemorrhagic shock rat model was established by bleeding from the femoral artery, which were randomly divided into the model group, epinephrine group (10 μg·kg^-1), YQFM low and high dose group (232.2 and 464.3 mg·kg^-1, 464.3 mg·kg^-1 was the clinical equivalent dose), and combined administration group (epinephrine 10 μg·kg^-1 + YQFM 464.3 mg·kg^-1), 10 in each group. Rats in the sham-operation group only recieved incision without bloodletting. After administration, the survival of rats within 3 h of administration was observed. An eight-channel non-invasive blood pressure meter was used to monitor systolic blood pressure before modeling, after modeling, and 3 h after drug administration. The levels of creatine kinase isoenzyme (CKMB), lactate dehydrogenase (LDH), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and superoxide dismutase (SOD) in serum were detected by enzyme-linked immunosorbent assay (ELISA). Results The mean arterial pressure of the rats was maintained at 30-40 mmHg after modeling, which indicated that the models were successfully established. All the rats in the shamoperation group and the treatment groups survived within 3 h, while three rats died in the model group, with a survival rate of 70% and an average survival time of (166.00±23.66) min. There was no significant difference in the basal blood pressure of rats in each group, but the systolic blood pressure of rats in the model group and each administration group was significantly lower than that in the sham-operation group after modeling (P<0.001). Compared with the model group, the systolic blood pressure in each drug administration group was significantly increased (P<0.001), and the pressor effect in the combined administration group was the best. Compared with the model group, the levels of serum CK-MB, LDH, ALT and AST in the epinephrine group were significantly decreased (P<0.01). The levels of LDH, ALT and AST in YQFM low-dose group were significantly decreased (P<0.05). The serum levels of CK-MB, LDH, ALT and AST in YQFM high-dose group and combined administration group were significantly decreased, while SOD level was significantly increased (P<0.05, 0.01, 0.001). Combined administration had the best effect on serum biochemical indexes. Conclusion YQFM can significantly increase systolic blood pressure of hemorrhagic shock rats and improve the level of related biochemical indexes in serum. The results of our research indicated that YQFM could exert a synergistic effect with epinephrine on the hemorrhagic shock rat.

R285.5