目的 基于灰色关联度法和偏最小二乘法研究芩苏胶囊抑菌作用的谱效关系。方法 使用高效液相色谱(HPLC)法建立10批芩苏胶囊的指纹图谱,采用管碟法测定芩苏胶囊(0.3 g·mL^-1)对5种常见致病菌(金黄色葡萄球菌、大肠埃希菌、铜绿假单胞菌、肺炎克雷伯菌、肺炎链球菌)的抑菌活性,使用灰色关联度分析法结合偏最小二乘法构建谱效关系。结果 10批样品指纹图谱中有37个共有峰,芩苏胶囊对5种致病菌均具有一定的抑菌作用,对金黄色葡萄球菌有较好的抑菌作用。6、10(迷迭香酸)、14、17(丹酚酸B)号峰为芩苏胶囊抑制金黄色葡萄球菌的主要成分;10(迷迭香酸)、17(丹酚酸B)、35(苦参碱)、37(氧化苦参碱)号峰为抑制大肠埃希菌的主要成分;6、7、8、9、10(迷迭香酸)、14、17(丹酚酸B)、27、31(丹参酮Ⅰ)、37(氧化苦参碱)号峰为抑制铜绿假单胞菌的主要成分;8、9、10(迷迭香酸)、14、17(丹酚酸B)、37(氧化苦参碱)为抑制肺炎克雷伯菌的主要成分;5、23(汉黄芩素)、27、30、31(丹参酮Ⅰ)、32、33(丹参酮Ⅱ_A)、34(槐果碱)、36(氧化槐定碱)、37(氧化苦参碱)号峰为抑制肺炎链球菌的主要成分。结论 通过谱效关系研究明确了芩苏胶囊抑菌药效作用是多种成分共同作用的结果,可为芩苏胶囊的药效物质基础研究与质量控制提供参考。

Objective To study the spectral effect relationship of antibacterial activity in Qinsu capsule based on grey correlation degree method and partial least square method. Methods Using HPLC to establish 10 Qinsu capsule of fingerprint, tube plate method is used to determine Qinsu capsule on five kinds of pathogenic bacteria (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pneumoniae) bacteriostatic activity, using grey correlation analysis method combined with partial least squares method to construct spectrum efficiency relations. Results There were 37 common peaks in the fingerprints of 10 batches of samples. Qinsu capsule had certain bacteriostatic effect on five kinds of pathogenic bacteria, and had better bacteriostatic effect on Staphylococcus aureus. Peak 6, 10 (rosinic acid), peak 14, peak 17 (salvianolic acid B) were the main components of Qinsu capsule against Staphylococcus aureus. Peak 10 (rosinic acid), peak 17 (salvianolic acid B), peak 35 (matrine), peak 37 (oxymatrine) were the main components of Qinsu capsule against Escherichia coli. Peaks 6, 7, 8, 9, 10 (rosinic acid), 14, 17 (salvianolic acid B), 27, 31 (tanshinone Ⅰ), 37 (oxymatrine) were the main components of Qinsu capsule against Pseudomonas aeruginosa. Peak 8, 9, 10 (rosinic acid), 14, 17 (salvianolic acid B), 37 (oxymatrine) were the main components of Qinsu capsule against Klebsiella pneumoniae. Peak 5, 23 (hanbaicalein), 27, 30, 31 (tanshinone Ⅰ), 32, 33 (tanshinone Ⅱ_A), 34 (sophorine), 36 (sophorine oxide), 37 (oxymatrine oxide) were the main components of Qinsu capsule against Streptococcus pneumoniae. Conclusion Through the study of spectrum effect relationship, it is clear that the antibacterial effect of Qinsu Capsule is the result of the joint action of multiple components, and this study can provide reference for the basic research and quality control of qinsu capsule.

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上海市科学技术委员会科研计划项目(19401901700)