目的 观察千金黄连片对高脂高糖致糖耐量异常模型大鼠的治疗作用。方法 选用4～6周龄的雄性SD大鼠90只，随机选取10只作为对照组，剩余大鼠喂饲高脂高糖饲料，连续喂饲5个月，测定对照组及模型组大鼠糖负荷（2.5 g·kg^-1）后0.5 h血糖值，挑选糖耐量异常的大鼠［0.5 h血糖值＞8.5 mmol·L^-1及0.5 h血糖值、血糖曲线下面积（AUC）均显著大于对照组］70只随机分为7组：模型组、盐酸二甲双胍片（125 mg·kg^-1）组、金芪降糖片（2.1 g·kg^-1）组、辛伐他汀组（0.8 mg·kg^-1）和千金黄连片高、中、低剂量（生药6.50、3.25、1.60 g·kg^-1）组，ig给药，给药体积10 mL·kg^-1，每天给药1次，连续给药28 d，对照组与模型组给予等量去离子水。观察千金黄连片给药1次对糖耐量的影响及给药多次对糖耐量、胰岛素耐量、淀粉耐量、血脂的影响：①糖耐量：禁食但不禁水12 h后，各组ig给予2.5 g·kg^-1的葡萄糖，分别测定给予葡萄糖前、给予葡萄糖后30、60、120 min各组大鼠血糖水平并计算AUC；②胰岛素耐量：动物不禁食，测定给胰岛素前血糖，各组sc给予5 U·kg^-1的胰岛素溶液，测定给予胰岛素后45、90、180 min各组大鼠的血糖水平并计算AUC；③淀粉耐量：禁食但不禁水16 h后，各组ig给予2.5 g·kg^-1的淀粉糊，分别测定给淀粉前、给予淀粉后30、60、120 min各组大鼠血糖水平。于连续给药4周后检测大鼠血清三酰甘油（TG）、总胆固醇（TC）、高密度脂蛋白-胆固醇（HDL-C）、低密度脂蛋白-胆固醇（LDL-C）水平。结果 与模型组比较，千金黄连片给药1次、给药1周、给药3周能显著降低高脂高糖致糖耐量异常大鼠糖负荷后血糖水平及AUC（P＜0.05、0.01、0.001） ；给药3周能使胰岛素降血糖作用显著增强（P＜0.05、0.01）；给药4周能显著降低淀粉负荷后血糖（P＜0.05、0.01），显著降低血清TG、LDL-C水平（P＜0.01、0.001）。结论 千金黄连片能显著地改善糖耐量异常；增强胰岛素敏感性，改善胰岛素抵抗；显著降低血清TG、LDL-C水平，改善脂质代谢异常。其降血糖作用与盐酸二甲双胍片相当，强于金芪降糖片，其改善胰岛素抵抗、增加胰岛素敏感性的作用强于盐酸二甲双胍片和金芪降糖片，调节血脂的作用与盐酸二甲双胍片、辛伐他汀相当。

Objective To observe the therapeutic effect of Qianjin Huanglian Pill (QJ) on prediabetes. Methods Ninety male SD rats aged from 4 to 6 weeks were selected and 10 were randomly selected as control group. The remaining rats were fed with high-fat and high-sugar diet for five months continuously. The blood glucose level at 0.5 h after glucose load (2.5 g·kg^-1) in control group and model group was measured. Seventy rats with abnormal glucose tolerance [0.5 h blood glucose>8.5 mmol·L^-1 and 0.5 h blood glucose and AUC were significantly higher than control group] were randomly divided into seven groups: Model group, Metformin hydrochloride tablet (125 mg·kg^-1) group, Jingqi Jiangtang tablet (2.1 g·kg^-1) group, simvastatin group (0.8 mg·kg^-1) and JQ highdose, medium-dose and low-dose (crude drug 6.50, 3.25, 1.60 g·kg^-1) groups, ig, 10 mL·kg^-1, once a day for 28 d, control group and model group were given the same amount of deionized water. To observe the effect of QJ once administration on glucose tolerance and the effect of multiple administration on glucose tolerance, insulin tolerance, starch tolerance and blood lipid. ① Glucose tolerance: After fasting for 12 h without water restriction, each group was given 2.5 g·kg^-1 glucose intragastrically, and the blood glucose levels were determined before, 30, 60 and 120 min after glucose administration, and the AUC was calculated. ② Insulin tolerance: Without fasting, blood glucose was measured before insulin administration, 5 U·kg^-1 insulin solution was sc in each group. Blood glucose levels of rats in 45, 90 and 180 min after insulin administration were measured and AUC was calculated. ③ Starch tolerance: After fasting but without water for 16 h, each group was given starch paste 2.5 g·kg^-1 intragaally, and blood glucose levels were determined before starch administration, 30, 60 and 120 min after starch administration. The levels of triglyceride (TG), total cholesterol (TC), high density lipoprotein-cholesterol (LDL-C) and low density lipoprotein-cholesterol (LDL-C) were detected in blood samples after four weeks of continuous administration. Results Compared with the model group, administration of QJ for once, one week and three weeks could significantly reduce blood glucose of rats after glucose loaded (P<0.05, 0.01, and 0.001). Administration of QJ for three weeks could significantly enhance the hypoglycemic effect of insulin (P<0.05 and 0.01). Administration of QJ for four weeks could significantly reduce blood glucose of rats after starch loaded (P<0.05 and 0.01) and serum TG and LDL levels (P<0.05 and 0.001). Conclusion QJ could significantly improve IGT, enhance insulin sensitivity, improve insulin resistance (IR), significantly reduce serum TG and LDL levels and improve abnormal lipid metabolism. Its hypoglycemic effect is comparable to that of metformin, which is stronger than that of Jinqi Jiangtang Tablet. Its effect of improving insulin resistance and increasing insulin sensitivity is stronger than that of metformin and Jingqi Jiangtang tablet. Its effect of regulating blood lipids is similar to that of metformin and simvastatin.

R285.5

国家科技重大专项(2019ZX09201005-001-002);国家中医药管理局中医药国际合作专项(0610-2140NF020630)