介绍了几种常见的血脑屏障体内外模型及一种脑渗透性分类方案。其中体外模型主要有溶剂水/分配模型、平行人工膜渗透模型、Transwell细胞模型、微流控芯片血脑屏障模型、永生化内皮细胞系建立的血脑屏障模型、三维血脑屏障模型等。体内模型主要有脑/血浆比率测定法、小鼠脑摄取量分析法、啮齿类动物原位脑灌注法、脑微透析法等。随着血脑屏障模型的不断成熟完善，将有助于筛选中枢神经系统药物，为神经系统疾病的治疗提供更多的理论依据。通过分析和评价各种不同的血脑屏障模型，以期为血脑屏障模型研究及中枢神经系统药物研发提供新的思路。

This article introduces several common in vivo and in vitro models of the blood-brain barrier and a classification scheme for brain permeability. Among them, in vitro models mainly include solvent water/distribution model, parallel artificial membrane permeation model, Transwell cell model, microfluidic chip blood-brain barrier model, blood-brain barrier model established by immortalized endothelial cell line, 3D blood-brain barrier model, etc. In vivo models mainly include brain/plasma ratio determination method, mouse brain uptake analysis method, rodent in situ brain perfusion method, brain microdialysis method and so on. As the blood-brain barrier model continues to mature and improve, it will help to screen drugs for the central nervous system and provide more theoretical basis for the treatment of neurological diseases. This article analyzes and evaluates various blood-brain barrier models, hoping to provide new ideas for blood-brain barrier model research and central nervous system drug research and development.

R965.3

国家重点研发计划——重大疾病治疗药物制剂质量研究评价技术联合研究（2020YFE0201700）