目的 探讨千金黄连方对2型糖尿病模型动物db/db小鼠血糖的影响。方法 C57BL/6J小鼠10只（雌雄各半）作为对照组，将db/db小鼠60只（雌雄各半）按血糖值随机分为6组：模型组、二甲双胍（0.25 g·kg^-1）组、金芪降糖片（4.2 g·kg^-1）组和千金黄连方高、中、低剂量（生药13.00、6.50、3.25 g·kg^-1）组。每天ig给药1次，给药体积20 mL·kg^-1，对照组及模型组ig给予等量去离子水。血糖仪测定千金黄连方给药1次对糖耐量、空腹血糖的影响及多次给药对空腹血糖、糖耐量、混合饮食餐后血糖、淀粉耐量（碳水化合物饮食餐后血糖）的影响；连续给药12周后，试剂盒法检测血清中糖化血清蛋白（GSP）、糖化血红蛋白（GHb）、胰岛素水平；小鼠放血处死后，剪开腹部，取腹部脂肪称质量，计算脂肪系数；HE染色后对肝组织进行病理学检查。结果 给药1次，与模型组比较，给药后1~2 h千金黄连方高剂量能显著降低小鼠空腹血糖值和糖耐量血糖值（P<0.05、0.01）。与模型组比较，多次给予千金黄连方能显著降低小鼠空腹血糖值、显著降低葡萄糖耐量血糖值、显著降低混合饮食餐后血糖值和淀粉耐量血糖值（P<0.05、0.01、0.01）。与模型组比较，千金黄连方高、中、低剂量组小鼠GHb、GSP及胰岛素水平均显著下降（P<0.05、0.01、0.001）；高、中剂量组小鼠腹部脂肪系数显著下降（P<0.05、0.01）；高、低剂量对肝脏空泡变性程度有减轻作用。结论 千金黄连方能显著降低db/db小鼠空腹血糖，改善糖耐量异常及增强胰岛素敏感性，显著减少腹部脂肪系数，对肝细胞的损伤也有一定的改善作用；其降血糖作用与二甲双胍相当，强于金芪降糖片，其改善胰岛素抵抗、增加胰岛素敏感性的作用强于二甲双胍和金芪降糖片。

Objective To investigate the effects of Qianjin Huanglian Prescription on blood glucose in db/db mouse of type 2 diabetes model. Methods Sixty db/db mice aged 4-6 weeks (half male and half female) were selected as the experimental group. Ten C57BL/6J mice aged 4-6 weeks (half male and half female) were selected as the control group. The db/db mice were randomly divided into six groups according to the blood glucose level, which were the model group, the metformin group, the Jinqi Jiangtang Tablets group, and the Qianjin Huanglian Prescription (raw medicine 13.00, 6.50, 3.25 g/kg). The effects of Qianjin Huanglian Prescription dosing once on glucose tolerance, fasting blood and The effects of Qianjin Huanglian Prescription multiple dosing on fasting blood glucose, glucose tolerance, mixed diet postprandial blood glucose, starch tolerance (carbohydrate diet postprandial blood glucose), serum glycated hemoglobin, glycosylated serum protein, insulin, abdominal fat weight and abdominal fat coefficient were measured separately.Pathological examination of liver tissues was also made. Results Dosing once, compared with the model group, the high dose of Qianjin Huanglian Prescription after 1-2 h after drug can significantly reduce the fasting blood glucose level and glucose tolerance of mice (P < 0.05 and 0.01). Multiple administration of Qianjin Huanglian Prescription can significantly reduce fasting blood glucose, improve glucose tolerance, improve Mixed diet postprandial blood glucose levels and carbohydrate diet postprandial blood glucose levels in mixed diets (P < 0.05, 0.01 and 0.001). Compared with model group, Qianjin Huanglian Prescription significantly reduce GHb and GSP levels, significantly reduce insulin level (P < 0.05, 0.01 and 0.001), improve insulin resistance; significantly reduce abdominal fat weight and abdominal fat coefficient in diabetic mice(P < 0.05 and 0.01), reduce the degree of Hepatic vacuolar degeneration. Conclusion Qianjin Huanglian Prescription can significantly reduce fasting blood glucose in db/db mice, improve impaired glucose tolerance and enhance insulin sensitivity, improve insulin resistance, and significantly reduce abdominal fat coefficient, There is also a certain improvement in the damage of hepatocytes in db/db mice. Its hypoglycemic effect is similar to metformin and stronger than Jinqi Jiangtang Tablets. Its effect of improving insulin resistance and increasing insulin sensitivity is stronger than that of metformin and Jinqi Jiangtang Tablets.

R285.5

天津市科技支撑计划重点项目（16YFZCSY00620）