目的 探讨灵芝孢子油、破壁灵芝孢子粉及灵芝孢子提取物对小鼠急性胃溃疡的影响。方法 雄性ICR小鼠随机分为12组：对照组，模型组，奥美拉唑（阳性药，4 mg·kg^-1）组，灵芝孢子提取物低、中、高剂量（0.2、0.4、0.8 g·kg^-1）组，破壁灵芝孢子粉低、中、高剂量（0.6、1.2、2.4 g·kg^-1）组，灵芝孢子油低、中、高剂量（0.2、0.4、0.8 g·kg^-1）组，每组20只。ig给药，每天1次，对照组和模型组给予等体积的生理盐水。连续给药7 d后，禁食12 h，除对照组外，每组随机选取10只小鼠ig无水乙醇（10 mL·kg^-1）建立急性胃溃疡模型，1.5 h后取材；每组余下10只小鼠ig吲哚美辛（40 mg·kg^-1）建立急性胃溃疡模型，3 h后取材。观察小鼠的一般状况、胃出血和溃疡情况，进行胃损伤评分，计算损伤抑制率和损伤发生率。结果 各组小鼠给药过程中无异常。小鼠给予无水乙醇后，模型组和奥美拉唑组10 min后开始出现行动变缓，肢体活动不协调，呼吸加深、频率减慢等症状，而给予灵芝孢子提取物、破壁灵芝孢子粉和灵芝孢子油的小鼠在30 min后才陆续出现症状；给予吲哚美辛的小鼠自主活动减少，各组之间无差别；对照组小鼠行为活动如常。与对照组比较，小鼠经无水乙醇、吲哚美辛ig导致非常明显的胃溃疡，胃损伤评分显著升高（P<0.01），损伤发生率为100%；与模型组比较，预防性给予灵芝孢子提取物、破壁灵芝孢子粉、灵芝孢子油可明显降低胃出血或溃疡等胃黏膜损伤，其中灵芝孢子提取物高剂量组、破壁灵芝孢子粉组、灵芝孢子油组的胃损伤评分显著降低（P<0.01），损伤抑制率显著提高（P<0.01），损伤发生率明显降低。结论 灵芝孢子油、破壁灵芝孢子粉及灵芝孢子提取物能有效抑制小鼠急性胃溃疡的发生，灵芝孢子油效果最佳，破壁灵芝孢子粉次之，并且对于无水乙醇导致的急性胃溃疡抑制作用尤为显著。

Objective To explore the influence of Ganoderma lucidum spores oil (GLSO), broken Ganoderma lucidum spore (BGLS) and Ganoderma lucidum spore extract (GLSE) on the formation of acute gastric ulcer. Methods Male ICR mice were randomly divided into 12 groups: control group, model group, omeprazole (4 mg·kg^-1) group, GLSE low, medium and high dose (0.2, 0.4, 0.8 g·kg^-1) groups, BGLS low, medium and high dose (0.6, 1.2, 2.4 g·kg^-1) groups, GLSO low, medium and high dose (0.2, 0.4, 0.8 g·kg^-1) groups, 20 in each group. Mice in control group and model group were given the same volume of normal saline. After seven days of continuous administration, ten mice in each group were randomly selected, except the control group, to establish acute gastric ulcer model intragastrically with anhydrous ethanol (10 mL·kg^-1), and the samples were taken 1.5 h later. Each group of the remaining 10 mice ig indomethacin (40 mg·kg^-1) to establish acute gastric ulcer models, the samples were collected after threehours. The general condition, gastric bleeding and ulcer of mice were observed, and the gastric injury score was performed, and the injury inhibition rate and injury incidence rate were calculated. Results There was no abnormality during administration in each group. After the mice were given anhydrous ethanol, the model group and omeprazole group began to show symptoms such as slowed movement, uncoordinated limb activity, deep breathing and slow frequency ten min later, while the mice given GLSO, BGLS and GLSE began to show symptoms 30 min later. The autonomic activity of indomethacin-induced model mice decreased in each group, and there was no difference among the groups. The behavior of mice in control group was as usual. Compared with control group, the gastric ulcer was induced by anhydrous ethanol and indomethacin intragastrically, and the gastric injury score was significantly increased (P < 0.01), and the injury rate was 100%. Compared with model group, preventive give GLSO, BGLS and GLSE can obviously reduce the bleeding or ulcer and gastric mucosa injury, stomach injury score significantly reduced (P < 0.01), the inhibition rate of damage increased significantly (P < 0.01), the injury rate was significantly reduced, in high dose group of GLSE, BGLS group and GLSO group. Conclusion BGLS and GLSE could suppress the formation of acute gastric ulcer in mice and the protective effect of GLSO on acute gastric ulcer was the best, followed by BGLS, and against significantly the alcoholic gastric ulcer.

R285.5