发育毒性是毒理学研究中最具挑战性的领域之一，传统的发育毒性评价方法均存在实验成本高、周期长、技术复杂等弊端。人多能干细胞（hPSC）包括人胚胎干细胞（hESC）和诱导多能干细胞（iPSC），具有分化多能性和无限增殖的潜力。近年来hPSC在发育毒性评价方面的研究逐渐增多，主要从hPSCs培养中代谢小分子的变化、hPSCs的定向分化、拟胚体的形态结构和转录组学变化、3D器官培养等方面综述了基于hPSC的药物发育毒性评价的最新研究进展，进而为今后药物发育毒性的研究提供参考。

Developmental toxicity is one of the most challenging fields in toxicology. The traditional developmental toxicity evaluation methods have the disadvantages of high cost, long cycle and complex technology. Human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSC), have the potential of pluripotent differentiation and unlimited proliferation. In recent years, there are more researches on hPSCs in developmental toxicity evaluation. This paper mainly reviews the latest research progress of hPSCs based developmental toxicity evaluation from the aspects of the changes of small metabolic molecules in hPSCs culture, the induced differentiation of hPSCs, the morphological structure and transcriptome changes of embryoid, and 3D organ culture, then provides reference methods for the study of drug developmental toxicity.

R965.3

国家自然科学基金资助项目（82074275）