目的 运用细胞膜色谱（CMC）技术检测分析人参皂苷与五味子木脂素中15种成分与血管内皮生长因子（VEGF）受体的作用。方法 运用Western blotting法检测筛选VEGF受体高表达细胞株；构建VEGF受体CMC，并建立ECV304/CMC online-LC检测方法，以索拉菲尼作为阳性药，对人参皂苷Rb₁、Rb₂、Rb₃、Rc、Rd、Rg₁、Rg₂、Rg₃、Rh₁、Ro、F₂，五味子甲素，五味子乙素，五味子醇甲，五味子醇乙进行VEGF受体结合强度的检测分析；CCK-8法检测筛选出的成分（5、10 μg/mL）对ECV304细胞的促增殖作用。结果 Western blotting结果显示，ECV304细胞中VEGF受体高表达；人参皂苷Rb₁、Rb₂、Rb₃、Rc、Rd、Rg₃、Rh₁、F₂、五味子甲素、五味子乙素与VEGF受体CMC柱有结合作用；CCK-8法检测以上成分对ECV304细胞的活力影响发现，人参皂苷Rb₂能促进细胞的增殖。结论 人参皂苷Rb₂能与VEGF受体结合，且显著促进ECV304细胞增殖。

Objective To detect and analyze the effect of 15 components from ginseng saponins and schisandra lignin on angiogenesis by cell membrane chromatography. Methods Western blotting detection was used to screen the cell lines with high expression of VEGF receptor. Cell membrane chromatographic column was prepared by ECV304 cell membrane and ECV304/CMC online-LC detection method was established. With sorafenib as a positive drug, the receptor binding strength of VEGF was detected and analyzed for ginsenosides Rb₁,Rb₂,Rb₃,Rc,Rd,Rg₁,Rg₂,Rg₃,Rh₁,Ro,F₂, deoxyschizandrin, γ -schizandrin, schisandrol A, schisandrol B. CCK-8 assay was used to detect the proliferative effects of the selected ingredients (5 and 10 μg/mL) on ECV304 cells. Results Western blotting results showed that VEGF receptor was highly expressed in ECV304 cells. It was found that ginsenoside Rb₁,Rb₂,Rb₃,Rc,Rd,Rg₃,Rh₁,F₂, schisandrin A and schisandrin B had binding effect. CCK-8 assay showed that ginsenoside Rb₂ could promote the proliferation of ECV304 cells. Conclusion Ginsenoside Rb₂ can bind to angiogenic VEGF receptor, and can significantly promote the proliferation of ECV304.

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