目的 采用大鼠大脑中动脉阻塞/再灌注（MCAO/R）模型考察注射用丹参多酚酸（SAFI）与丁苯酞氯化钠注射液（BSCI）联合使用对脑缺血再灌注大鼠的治疗作用。方法 构建大鼠MCAO/R模型，术后24 h根据神经功能缺损评分将大鼠平均分为：模型组、SAFI（11.52 mg/kg，每天给药1次）组、BSCI（8.86 mL/kg，以丁苯酞计为2.22 mg/kg，每天给药2次）组、SAFI（11.52 mg/kg，每天给药1次）＋BSCI（2.22 mg/kg，每天给药2次）组，每组20只。各组大鼠均于分组后立即尾iv给药，连续给药14 d。给药体积均为10 mL/kg，假手术组和模型组给予0.9%的氯化钠注射液。考察给药后各组大鼠神经功能缺损评分；ELISA法检测缺血半脑组织中白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）和超氧化物歧化酶（SOD）的含量；TTC染色检测脑梗死面积；HE染色观察脑组织病理变化。结果 与给药前及模型组比较，SAFI和SAFI＋BSCI组神经功能缺损评分显著降低（P<0.05）；与模型组比较，3个给药组大鼠脑组织IL-6、IL-1β和TNF-α水平均显著降低，SOD水平显著升高（P<0.05、0.01）； 3个给药组的大鼠脑梗死面积均显著减小（P<0.01、0.001），其中SAFI＋BSCI组的梗死面积最小； 3个给药组脑组织和细胞坏死的现象均减轻，其中SAFI和SAFI＋BSCI组表现出更少的炎性浸润和瘀血现象。结论 SAFI、BSCI和SAFI＋BSCI均能显著改善缺血性脑卒中大鼠的神经损伤，SAFI和BSCI联用有一定的药效协同作用。

Objective To investigate the synergistic effect of Salvianolic Acid for Injection (SAFI) combined with Butylphthalide and Sodium Chloride Injection (butylphthalide) by middle cerebral artery occlusion/reperfusion (MCAO/R) model. Methods The rat MCAO/R model was established, and 24 h after surgery, the rats were divided into:model group, SAFI (11.52 mg/kg, once a day) group, BSCI (2.22 mg/kg, twice a day) group, SAFI (11.52 mg/kg, once a day) + BSCI (2.22 mg/kg, twice a day) group, 20 in each group. Rats in all groups were given tail-IV medication immediately after grouping for 14 consecutive days. The volume of administration was 10 mL/kg, and the sham operation group and model group were given 0.9% sodium chloride injection. The levels of interleukin-1 β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and superoxide dismutase (SOD) in ischemic cerebral tissue were determined by ELISA and neurological deficit score after administration. The area of cerebral infarction was detected by TTC staining. HE staining was used to observe the pathological changes of brain tissue. Results Compared with the same group before administration and model group, neurological deficit score in SAFI and SAFI + BSCI groups was significantly decreased (P<0.05). Compared with model group, the levels of IL-6, IL-1β and TNF-α in brain tissue of rats in three drug treatment groups were significantly decreased, while SOD level was significantly increased (P<0.05, 0.01). The cerebral infarction area of rats in the three drug administration groups was significantly decreased (P<0.01, 0.001), and the SAFI+BSCI group had the smallest infarct area. Tissue and cell necrosis were reduced in all three groups, and the SAFI and SAFI+BSCI groups showed less inflammatory infiltration and congestion. Conclusion SAFI, BSCI and SAFI + BSCI can significantly improve nerve injury in ischemic stroke rats. The combination of SAFI and BSCI has better efficacy.

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