目的 考察新绿原酸对宫颈癌HeLa细胞体外迁移模型和斑马鱼移植瘤模型的影响。方法 MTT法检测新绿原酸（20、40、80、160、320 μmol/L）对HeLa细胞增殖的作用；应用宫颈癌细胞HeLa划痕愈合模型和Transwell实验考察新绿原酸（28、56、113 μmol/L）对体外迁移作用的影响；同时利用构建的绿色荧光蛋白（GFP）的HeLa细胞系建立斑马鱼移植瘤模型，考察新绿原酸（56、113、226 μmol/L）对体内移植瘤模型细胞迁移的作用；Western blotting法检测新绿原酸对内皮生长因子（EGF）诱导的HeLa细胞中表皮生长因子受体（EGFR）、p-EGFR蛋白表达的影响。结果 MTT结果显示，新绿原酸在160 μmol/L及以下浓度对HeLa细胞增殖无明显影响；与对照组比较，56、113 μmol/L新绿原酸显著抑制划痕愈合，抑制率分别为49.3%（P<0.05）和64.1%（P<0.01）；Transwell实验结果提示，113 μmol/L新绿原酸具有显著抑制HeLa细胞向下层迁移的作用（P<0.05）；与模型组比较，113、226 μmol/L新绿原酸可显著减少斑马鱼移植瘤模型的HeLa细胞的迁移面积（P<0.05、0.01）；Western blotting结果显示，与EGF组比较，新绿原酸对EGFR表达和磷酸化有明显抑制作用，56、113 μmol/L浓度组差异显著（P<0.01）。结论 在未影响HeLa细胞增殖的浓度下，新绿原酸具有抑制宫颈癌HeLa细胞迁移的作用，其作用机制与抑制EGFR的表达有关。

Objective To investigate the effect of 5-caffeoylquinic acid (5-CQA) on migration of human cervical cancer HeLa cells. Methods MTT assay was used to detect the effect of 5-CQA (20, 40, 80, 160, 320 μmol/L) on the proliferation of HeLa cells. The wound healing model of HeLa cells and Transwell were used to detect the migration of 5-CQA (28, 56, 113 μmol/L) in vitro. At the same time, zebrafish xenograft tumor model was established by using the constructed HeLa cell line of green fluorescent protein (GFP), and the migration of 5-CQA (56, 113, 226 μmol/L) was detected. Western blotting was used to detect the effect of 5-CQA on the expression of p-epidermal growth factor receptor (EGFR) and EGFR protein in HeLa cells induced by EGF. Results MTT results showed that there was no significant effect on the proliferation of HeLa cells of 5-CQA at the concentration of 160 μmol/L or below. Compared with control group, 56, 113 μmol/L 5-CQA significantly inhibited wound healing with inhibition rates of 49.3% (P < 0.05) and 64.1% (P < 0.01), respectively. Transwell results showed that 113 μmol/L 5-CQA significantly inhibited the migration of HeLa cells (P < 0.05). Compared with model group, 113, 226 μmol/L 5-CQA significantly reduced the migration area of HeLa cells (P < 0.05, 0.01). Western blotting results showed that compared with EGF group, 5-CQA (56 and 113 μmol/L) significantly inhibited EGFR expression and phosphorylation (P < 0.01). Conclusions 5-CQA can inhibite cervical cancer HeLa cell migration by blocking EGFR expression.

R285.5;R737.3;R332

国家重点研发计划（2018YFC1707300）；山东省重大科技创新工程项目（2019JZZY020905）；齐鲁工业大学（山东省科学院）科教产融合创新试点工程项目（2020KJC-ZD08）