目的 评价二甲基姜黄素、黄芩苷、紫檀茋对异烟肼导致斑马鱼神经损伤的保护作用。方法 挑选受精后2 h（2hpf）的斑马鱼胚胎，设置对照组（胚胎培养用水处理）、异烟肼组（4 mmol/L）、二甲基姜黄素（0.5、1.0、2.0、4.0 μmol/L）组、黄芩苷（0.1、0.2、0.5、1.0 μmol/L）组、紫檀茋（0.5、1.0、2.0、4.0 μmol/L）组，异烟肼分别与不同浓度的药物共处理120 h，用Zebrabox斑马鱼行为分析仪分析斑马鱼幼鱼行为学轨迹变化，荧光显微镜下观察斑马鱼幼鱼多巴胺神经元的发育情况。结果 与对照组比较，异烟肼组运动轨迹明显减少，运动总距离和低速、中速运动距离均显著下降（P<0.01），运动速度明显降低；神经节长度显著降低（P<0.01）。与异烟肼组比较，二甲基姜黄素组运动轨迹明显增加，斑马鱼运动状态主要为低速运动，各浓度的二甲基姜黄素组斑马鱼总运动距离和低速运动距离均显著增加（P<0.01），运动速度明显增加，0.5、2.0、4.0 μmol/L的二甲基姜黄素组中速运动距离显著增加（P<0.01）；黄芩苷组运动轨迹明显增加，0.1、0.2、0.5和1.0 μmol/L的黄芩苷组斑马鱼总运动距离和低速运动距离均显著增加（P<0.01），运动速度明显增加；0.5和1.0 μmol/L紫檀茋组运动轨迹明显增加，紫檀茋0.5 μmol/L组的总运动距离和低速运动距离均显著增加（P<0.01），紫檀茋0.5和1.0 μmol/L浓度组运动速度明显增加。与异烟肼组比较，二甲基姜黄素和黄芩苷各浓度组多巴胺相对神经节长度显著增加（P<0.01）；紫檀茋1 μmol/L组相对神经节长度显著增加（P<0.01）。结论 二甲基姜黄素、黄芩苷和0.5、1.0 μmol/L紫檀茋对异烟肼导致的斑马鱼神经损伤有明显保护作用。

Objective To evaluate the protective effects of dimethyl curcumin, baclidin and pterostilbene on isoniazid induced nerve damage in zebrafish. Methods 2 hpf (hours post fertilization) zebrafish embryos were used in the experiments. These fish were respectively treated with control group, isoniazid group, and isoniazid along with different concentrations of dimethyl curcumin (0.5, 1.0, 2.0, 4.0 μmol/L), bacitrin (0.1, 0.2, 0.5, 1.0 μmol/L) and pterostilbene (0.5, 1.0, 2.0, 4.0 μmol/L) co-treatment group. At 120 h, the Zebrabox was used to analyze the behavioral trajectory changes of zebrafish, and the development of dopaminergic neurons in zebrafish larvae was observed under a fluorescence microscope. Results Compared with control group, the movement track of isoniazid group was significantly reduced, the total movement distance and the low and medium speed movement distance were significantly decreased (P < 0.01), and the movement speed was significantly reduced, and the ganglion length was significantly decreased (P < 0.01). Compared with isoniazid group, the movement track of dimethylcurcumin group was significantly increased, and the zebrafish movement state was mainly low speed movement. The total movement distance and low speed movement distance of each concentration of dimethylcurcumin group were significantly increased (P < 0.01), and the movement speed was significantly increased, the distance of middle speed movement was significantly increased in dimethylcurcumin 0.5, 2.0, 4.0 μmol/L group (P < 0.01). In the baicalin group, the trajectories increased significantly, the total movement distance and low speed movement distance of zebrafish were significantly increased (P < 0.01) and the movement speed was significantly increased (P < 0.01). The trajectories increased significantly in pterostilbene 0.5 and 1.0 μmol/L group, the total movement distance and low speed movement distance of pterostilbene 0.5 μmol/L group were significantly increased (P < 0.01) and the movement speed was significantly increased in pterostilbene 0.5 and 1.0 μmol/L group. Compared with isoniazid group, the relative ganglion length of dopamine in dimethylcurcumin, baicalin and pterostilbene 1 μmol/L groups was significantly increased (P < 0.01).Conclusion Dimethylcurcumin, baclidin and lower-concentration pterostilbene have obvious protective effects on isoniazid-caused zebrafish nerve injury.

山东省优秀青年人才基金项目（ZR2020YQ60）；齐鲁工业大学（山东省科学院）科教产融合创新试点工程项目（2020KJCZD08）；济南市“高校20条”资助项目（2020GXRC053）