[关键词]
[摘要]
目的 探讨香砂平胃颗粒对湿阻中焦证大鼠胃肠功能的影响及机制。方法 将大鼠随机分成对照组、模型组、莫沙必利组(1.35 mg/kg)、洛哌丁胺组(0.36 mg/kg)和香砂平胃颗粒低、中、高剂量(0.9、1.8、3.6 g/kg)组,除对照组外,内湿(定期ig猪油、蜂蜜水)、外湿(游泳、放在潮湿垫料中饲养)结合制备湿阻中焦证动物模型,模型成功后,连续ig给药7 d。观察大鼠一般状态,采用称质量法计算大鼠胃残留率,小肠炭末推进法计算大鼠肠推进率,并利用酶联免疫吸附法检测大鼠血清中胃动素、胃泌素、生长抑素和P-物质,以及炎性因子肿瘤坏死因子(TNF)-α和白细胞介素(IL)-10的水平,同时利用实时荧光定量RCR(qRT-PCR)技术检测大鼠结肠水通道蛋白3(AQP3)mRNA转录水平的变化。结果 与对照组比较,模型组大鼠体质量增长明显减缓,摄食量、饮水量均减少,毛色发黄、乏力、呆滞、嗜睡、大便黏腻;给予香砂平胃颗粒治疗7 d后,一般情况有所恢复,摄食量增加,体质量增长加快,大便性状有所好转。与模型组比较,香砂平胃颗粒中、高剂量组大鼠胃排空率显著增加,高剂量组小肠炭墨推进率显著增加(P<0.01、0.001);中、高剂量组大鼠的胃动素、胃泌素和P-物质水平显著增加(P<0.01、0.001),高剂量组生长抑素水平显著降低(P<0.01);中、高剂量组血清TNF-α水平显著降低(P<0.01、0.001);高剂量组大鼠的IL-10水平显著增高(P<0.01);中、高剂量组大鼠的AQP3mRNA转录水平显著上调(P<0.05、0.001)。结论 香砂平胃颗粒可以改善湿阻中焦证大鼠的胃肠功能,机制可能与增强胃肠运动、增加胃肠激素分泌、抑制湿阻所致的消化道炎症以及促进水分从肠腔的转运相关。
[Key word]
[Abstract]
Objective To study the effects of Xiangsha Pingwei Keli for the syndrome of damp retention in middle-jiao. Methods The rats were divided into control group, model group, mosapride (1.35 mg/kg) group, loperamide (0.36 mg/kg) group, Xiangsha Pingwei Keli low, middle and high dose (0.9, 1.8, and 3.6 g/kg) group. The rats were treated for 7 days. The gastric residual rate was calculated by weighing method, and the intestinal propulsion rate was calculated by small intestinal charcoal pushing method. The contents of motilin, gastrin, somatostatin, substance P, TNF-α and IL-10 in serum were detected by ELISA. At the same time, the expression of AQP3 mRNA was detected by qRT-PCR. Results Compared with control group, the growth of body weight of rats in model group was significantly slowed down, the amount of food intake and water intake were reduced, the hair color was yellow, fatigue, lethargy, drowsiness, and feces were sticky. After 7 days of treatment with Xiangsha Pingwei Keli, the general condition was somewhat recovered, the food intake increased, the body weight increased faster, and the fecal characteristics were improved. Compare with model group. The gastric residual rate of rats in Xiangsha Pingwei Keli medium and high-dose groups was significantly increased, and the intestinal carbon ink propelling rate in the high-dose group was significantly increased (P<0.01 and 0.001). The levels of motilin, gastrin and P-substance in middle and high dose groups were significantly increased (P<0.01 and 0.001), while the levels of somatostatin in high dose group were significantly decreased (P<0.01). Serum TNF-α level in medium and high dose groups was significantly decreased (P<0.01 and 0.001); The level of IL-10 in high-dose group was significantly increased (P<0.01); The transcription level of AQP3 mRNA in medium and high dose groups was significantly up-regulated (P<0.05 and 0.001). Conclusion The mechanism of Xiangsha Pingwei Keli in the treatment of rats with damp retention in middle-jiao syndrome may be to enhance gastrointestinal motility, increase the secretion of gastrointestinal hormones, inhibit gastrointestinal inflammation and promote water transport from intestinal cavity.
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[基金项目]
“十三五”国家重点研发计划项目(2018YFC1602104)