目的探讨刺槐素对肺癌细胞增殖、迁移和侵袭的影响及分子机制。方法用浓度分别为4、8、16 μmol/L的刺槐素处理A549细胞，作为低、中、高浓度组，不作任何处理的细胞作为对照组；将pcDNA、pcDNA-LZTFL1转染至A549细胞中，记为pcDNA组、pcDNA-LZTFL1组；将si-NC、si-LZTFL1转染至A549细胞中再用16 μmol/L的刺槐素处理，记为刺槐素+si-NC组、刺槐素+si-LZTFL1组。四甲基偶氮唑盐比色法（MTT）检测细胞增殖抑制率；Western blotting法检测LZTFL1、CyclinD1、p21、MMP-2、MMP-9蛋白表达；Transwell检测细胞迁移和侵袭；实时荧光定量PCR（qRT-PCR）检测LZTFL1 mRNA表达水平。结果与对照组比较，刺槐素低、中、高浓度处理的肺癌A549细胞中细胞增殖抑制率显著升高，细胞迁移、侵袭数显著降低，CyclinD1、MMP-2、MMP-9蛋白表达水平显著降低，p21蛋白表达水平显著升高，LZTFL1 mRNA和蛋白表达水平显著升高（P<0.05）。过表达LZTFL1抑制肺癌细胞的增殖、迁移和侵袭。干扰LZTFL1表达逆转了刺槐素对肺癌A549细胞增殖、迁移和侵袭的抑制作用。结论刺槐素可能通过上调LZTFL1的表达抑制肺癌细胞的增殖、迁移和侵袭。

Objective To investigate the effect of acacetin on the proliferation, migration and invasion of lung cancer cells and its molecular mechanism. Methods A549 cells were treated with acacetins at concentrations of 4, 8 and 16 μmol/L, respectively, as the low, medium, and high concentration groups of acacetin, and cells without any treatment were used as control groups; pcDNA, pcDNA-LZTFL1 was transfected into A549 cells and recorded as pcDNA group and pcDNA-LZTFL1 group; si-NC and si-LZTFL1 were transfected into A549 cells and treated with 16 μmol/L acacetin and recorded as acacetin+si-NC group, acacetin+si-LZTFL1 group. Tetramethylazozolium colorimetry (MTT) was used to detect cell proliferation inhibition rate; Western blotting was used to detect LZTFL1, CyclinD1, p21, MMP-2, and MMP-9 protein expression; Transwell was used to detect cell migration and invasion; real-time fluorescent quantitative PCR (RT-qPCR) was used to detect the expression of LZTFL1 mRNA. Results Compared with the control group, the cell proliferation inhibition rate of lung cancer A549 cells treated with low, medium, and high concentrations of acacetin was significantly increased, the number of cell migration and invasion was significantly reduced, and the expressions of CyclinD1, MMP-2 and MMP-9 proteins were significantly reduced, the expression of p21 protein was significantly increased, LZTFL1 mRNA and protein expression levels were significantly increased (P < 0.05). LZTFL1 overexpression inhibits the proliferation, migration and invasion of lung cancer cells. Interfering with LZTFL1 expression reversed the inhibitory effect of acacetin on the proliferation, migration and invasion of lung cancer A549 cells. Conclusions Acacetin may inhibit the proliferation, migration and invasion of lung cancer cells by up-regulating the expression of LZTFL1.

R965