[关键词]
[摘要]
目的探讨大蒜素联合顺铂对人宫颈癌HeLa细胞增殖的抑制作用并探讨其机制。方法取对数生长期HeLa细胞,设对照组、大蒜素(50 μg/mL)组、顺铂(5 μg/mL)组、联合给药组(大蒜素50 μg/mL+顺铂5 μg/mL)。药物干预48 h后,通过MTT法检测细胞增殖抑制率,运用CompuSyn软件计算大蒜素与顺铂联合指数(CI),流式细胞术检测细胞周期分布和细胞凋亡水平,Western blotting法检测Cyclin D1、CDK2、P27、Cleaved Caspase-3、Bcl-2、Bax蛋白表达。结果与大蒜素组和顺铂组比较,联合给药组HeLa细胞增殖抑制率显著升高(P<0.05、0.01),CI为0.69(提示大蒜素与顺铂具有中度协同效应)。与对照组比较,大蒜素组和顺铂组G0/G1期细胞比例和细胞凋亡率显著升高(P<0.05、0.01),Cyclin D1、CDK2、Bcl-2表达显著下调且P27、Cleaved Caspase-3、Bax表达显著上调(P<0.05、0.01),Bax/Bcl-2比值显著升高(P<0.01)。与大蒜素组和顺铂组比较,联合给药组G0/G1期细胞比例和细胞凋亡率显著升高(P<0.05、0.01),Cyclin D1表达显著下调且P27、Cleaved Caspase-3、Bax表达显著上调(P<0.05、0.01),Bax/Bcl-2值显著升高(P<0.01)。结论大蒜素联合顺铂具有协同抑制人宫颈癌HeLa细胞增殖的作用,可能与调节细胞周期和凋亡相关蛋白表达,进而阻滞细胞周期进程并促进细胞凋亡有关。
[Key word]
[Abstract]
Objective To investigate the inhibitory effect of allicin combined with cisplatin on the proliferation of human cervical cancer HeLa cells and explore its mechanism. Methods HeLa cells in logarithmic growth phase were set control group (culture solution only), allicin group (50 μg/mL), cisplatin group (5 μg/mL) and combination group (50 μg/mL allicin + 5 μg/mL cisplatin). 48 h after given drugs, the proliferation inhibition rate of HeLa cells was detected by MTT method, the allicin and cisplatin combination index (CI) was calculated by CompuSyn software; the cell cycle distribution and apoptosis level were detected by Flow Cytometry; the expression of Cyclin D1, CDK2, P27, Cleaved Caspase-3, Bcl-2, Bax proteins were detected by Western blotting. Results Compared with allicin group and cisplatin group, the proliferation inhibition rate of combination group was significantly increased (P < 0.05 or 0.01), CI was 0.69 (which suggested that allicin and cisplatin have moderate synergistic effect). Compared with control group, the cell ratio in G0/G1 phase and the apoptosis rate in allicin group and cisplatin group were significantly increased (P < 0.05 or 0.01), the expressions of Cyclin D1, CDK2, Bcl-2 were significantly decreased and P27, Cleaved Caspase-3, Bax were significantly increased (P < 0.05 or 0.01), the ratio of Bax/Bcl-2 was significantly increased (P < 0.01). Compared with allicin group and cisplatin group, the cell ratio in G0/G1 phase and the apoptosis rate in combination group were significantly increased (P < 0.05 or 0.01), the expressions of Cyclin D1 was significantly decreased and P27, Cleaved Caspase-3, Bax were significantly increased (P < 0.05 or 0.01), the ratio of Bax/Bcl-2 was significantly increased (P < 0.01). Conclusions Allicin combined with cisplatin has the synergistically effects on inhibiting the proliferation of human cervical cancer HeLa cells; which may be related to the regulation of cell cycle and apoptosis-related protein expression, thereby blocking cell cycle progress and promoting cell apoptosis.
[中图分类号]
R965.1
[基金项目]
河北省医学科学研究重点课题计划(20181831)