目的 通过网络药理学的方法，筛选黄芩Scutellariae Radix的主要活性成分，构建药物-成分-疾病-靶点网络，探讨其治疗鼻咽癌分子机制。方法 采用TCMSP、String及Genecard等数据库，获取黄芩化学成分及其相关靶点、鼻咽癌疾病的靶点，并制作蛋白互作网络。通过Cytoscape 3.7.2绘制药物-成分-疾病-靶点网络。基于生物信息学开源软件Bioconductor以及R统计程序设计语言对黄芩作用的生物学过程以及KEGG进行富集分析。结果 黄芩的36个有效成分通过调控48个靶点和99条通路对鼻咽癌进行治疗，4个关键的化合物为汉黄芩素、黄芩素、刺槐素及β-谷甾醇，可通过AKT1、JUN、CASP3、VEGFA等关键靶蛋白介导AGE-RAGE、乙型肝炎、白细胞介素17等信号通路发挥抗鼻咽癌作用。结论 黄芩可以通过多靶点、多途径参与鼻咽癌的治疗。

Objective To investigate the potential therapy targets and pharmacological mechanism of Scutellariae Radix in nasopharyngeal carcinoma based on network pharmacology. Methods Drug ingredients-target network was constructed based on the target sets of Scutellariae Radix and nasopharyngeal carcinoma. The overlapping genes between Scutellariae Radix targets and nasopharyngeal carcinoma-related molecular targets were investigated using Cytoscape3.7.2. Furthermore, the PPI network and module was constructed based on overlapping genes and hub nodes, respectively, followed by the pathway enrichment analysis. Results The 36 active components of Scutellariae Radix can regulate 48 targets and 99 pathways to treat nasopharyngeal carcinoma. Four key compounds are wogonin, baicalein, robinia pseudoacacia and β sitosterol. They can mediate AGE-RAGE, hepatitis B and interleukin-17 signaling pathways through AKT1, Jun, CASP3 and VEGFA. Conclusion Scutellariae Radix can participate in the treatment of nasopharyngeal carcinoma through multiple targets and pathways.

R285.5