[关键词]
[摘要]
目的 建立白及Bletilla striata(Thunb.) Reichb.f.药材UPLC指纹图谱,分析云南、贵州产白及药材大小与化学成分间的关联关系,为两地白及药材的质量评价提供依据。方法 依据白及单个块茎的质量将云南、贵州产白及划分为大(质量≥ 10 g)、中(5 g ≤质量<10 g)、小(质量<5 g)3类,建立以儿茶素为内标、可进行半定量分析的白及UPLC指纹图谱测定方法,测定两产地不同大小的白及指纹图谱。通过对指纹图谱的相似度分析、聚类分析、热图分析,Spearman相关性分析,探讨两产地白及药材大小与化学成分间的关系。结果 云南、贵州两地的UPLC指纹图谱共有14个共有峰;17批云南产白及的相似度均在0.85以上,22批贵州产白及的相似度均在0.8以上,而两产地对照指纹图谱间的相似度较低(0.760),且云南产白及谱图缺少16、17号峰;将两产地相同规格的样品进行比较,贵州产白及的共有峰成分含量大于云南产白及;两产地指纹图谱中1、2、5、8、9、10、12、13、15号共有峰化学成分含量与白及大小呈负相关(P<0.05,0.4<r ≤ 0.8),其中8、9、10号峰与白及大小的相关性较强(r>0.7)。结论 云南和贵州产地白及药材中化学成分及其含量存在明显差异,且同一产地白及大小与1、2、5、8、9、10、12、13、15号峰化学成分呈负相关。除外在性状外,建议考虑将产地作为白及药材规格等级划分的指标之一,为两地白及药材质量优劣的评价提供参考。
[Key word]
[Abstract]
Objective To establish the UPLC fingerprints for analyzing the relationship between the size and chemical constituents content of Bletillae Rhizoma from Yunnan and Guizhou, and provide a basis for quality control of Bletillae Rhizoma. Methods Bletillae Rhizoma collected from Yunnan and Guizhou were divided into three categories (large:m ≥ 10 g, medium:5 g ≤ m < 10 g and small:m < 5 g) based on the tuber weight. The UPLC fingerprints were developed by using catechin as an internal standard, which can be used for semi-quantitative analysis. Similarity analysis, cluster analysis, heat map analysis, and Spearman correlation analysis were used to analyze the relationship between the size and chemical constituents content of Bletillae Rhizoma from the two origins. Results The 14 common peaks were marked in the fingerprint of Bletillae Rhizoma. The similarity of 17 batches of Bletillae Rhizoma from Yunnan was above 0.85, and the similarity of 22 batches of Bletillae Rhizoma from Guizhou was above 0.8. The similarity of the control fingerprints between the two regions was lower as 0.760, and the fingerprints of Bletillae Rhizoma produced in Yunnan lacked peaks 16 and 17. The content of the same components in samples from Guizhou were usually higher than that from Yunnan with the same size. The content of the most common peaks (No. 1, 2, 5, 8, 9, 10, 12, 13, and 15) in the fingerprints is negatively correlated with the size of Bletillae Rhizoma (P<0.05, 0.4 < r ≤ 0.8), and peaks 8, 9, and 10 have a strong correlation with the size of Bletillae Rhizoma (r>0.7). Conclusion There are obvious differences in the chemical constituents of Bletillae Rhizoma from Yunnan and Guizhou. And the size of Bletillae Rhizoma in the same origin has a negative correlation with the chemical constituents content. Therefore, in addition to the size of Bletillae Rhizoma, the origin of Bletillae Rhizoma should also be considered as one of the factors for the quality classification of Bletillae Rhizoma, which provides a reference for the evaluation of the quality of Bletillae Rhizoma from Yunnan and Guizhou.
[中图分类号]
R917
[基金项目]
国家发改委中医药管理局中药标准化项目(ZYBZH-Y-HUB-20)