目的 建立SD大鼠代谢相关脂肪性肝病（metabolic associated fatty liver disease，MAFLD）模型，观察大鼠病理变化。方法 用高糖高脂饲料饲喂雄性SD大鼠20周，检测模型组大鼠血清和肝匀浆相关生化指标的水平、肝脏脏器质量和系数、肝脏组织病理学变化。结果 模型组大鼠饲喂高糖高脂饲料20周后，与对照组比较，血清总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）及肝脏TC和三酰甘油（TG）的水平均显著升高（P<0.01），血清TG、高密度脂蛋白胆固醇（HDLC）的水平均显著降低（P<0.01），肝脏质量及系数显著性增加（P<0.01）。肝组织的病理改变为严重肝细胞脂肪变性及多灶性肉芽肿性炎。结论 高糖高脂饲料饲喂成功建立了合适的MAFLD动物模型，肝脏病理改变可见广泛的脂肪变性和大量的肉芽肿性炎症，未见纤维化。

Objectives To establish a model of metabolic associated fatty liver disease (MAFLD) in Sprague-Dawley rats. Methods 30 5-week-old male Sprague-Dawley rats in the model group were fed with high-sucrose-fat diet for 20 weeks, another 30 rats in the control group were fed with normal diet. The following indicators were evaluated:3 biochemical indexes in the serum and liver homogenate, organ weight and ratio, histopathological changes of the liver. Results Compared with the control group, the rats in model group showed significant increase in the level of total cholesterol (TC), triglyceride (TG), low density lipoprotein chesterol (LDL-C) and high density lipoprotein chesterol (HDL-C) in serum or liver homogenate. Also, the organ weight and ratio of liver in model group were increased significantly. Moreover, severe hepatocyte steatosis and multifocal granulomatous inflammation were observed in the livers of model group. Conclusions An appropriate rat model of MAFLD was successfully established by fed with high-sucrose-fat diet for 20 weeks. Severe hepatocyte steatosis and multifocal granulomatous inflammation were observed in the livers of model group and no fibrosis were found.

R965.1

国家科技重大专项重大新药创制（2018ZX09721004-001-003）