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[摘要]
目的 探究甘草次酸对胃癌SGC7901细胞增殖、凋亡及PI3K-Akt-mTOR通路的影响。方法 体外培养胃癌SGC7901细胞,分为对照组和甘草次酸低、中、高浓度(12.5、25.0、50.0 μmol/L)组,药物处理24、48、72 h后,CCK-8法检测细胞增殖情况;药物处理48 h后,流式细胞仪检测细胞周期分布及细胞凋亡情况;Western blotting检测凋亡相关蛋白Bcl-2、Bax、cleaved Caspase-3以及PI3K-Akt-mTOR信号通路中磷酸化PI3K、Akt、mTOR蛋白表达水平。结果 甘草次酸低、中、高浓度组胃癌SGC7901细胞增殖受到明显抑制,并呈时间和剂量相关性(P<0.05)。与对照组比较,甘草次酸低、中、高浓度组胃癌SGC7901细胞G0/G1期细胞比例显著降低(P<0.05),G2/M期细胞比例及凋亡率显著升高(P<0.05),Bax、cleaved Caspase-3蛋白表达水平显著升高(P<0.05),Bcl-2、p-PI3K、p-Akt、p-mTOR蛋白表达水平显著降低(P<0.05)。结论 甘草次酸可抑制胃癌SGC7901细胞增殖,促进其凋亡,其机制可能与抑制PI3K-Akt-mTOR信号通路激活有关。
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[Abstract]
Objective To investigate the effects of glycyrrhetinic acid on proliferation, apoptosis and phosphatidylinositol 3-kinase (PI3K) -serine/threonine protein kinase (Akt) -mammalian target of rapamycin (mTOR) (PI3K-Akt-mTOR) pathway of gastric cancer SGC7901 cells. Methods Gastric cancer SGC7901 cells were cultured in vitro, and divided into control group, low, medium and high concentration (12.5, 25.0 and 50.0 μmol/L) groups of glycyrrhetinic acid. Cells proliferation was detected by CCK-8; Cells cycle distribution and apoptosis were detected by flow cytometry; The apoptosis-associated proteins Bcl-2, Bax, cleaved Caspase-3, and phosphorylation of PI3K, Akt and mTOR protein PI3K-Akt-mTOR signaling pathway expression levels were analysed by Western blotting. Results The proliferation of gastric cancer SGC7901 cells in low, medium and high concentrations groups of glycyrrhetinic acid was significantly inhibited in a time- and dose-dependent manner (P<0.05). Compared with the control group, in the low, middle and high concentration groups of glycyrrhetinic acid, the proportion of G0/G1 phase cells of gastric cancer SGC7901 cells significantly decreased (P<0.05), and the proportion of G2/M phase cells and the apoptosis rate significantly increased (P<0.05). The expression levels of Bax and cleaved Caspase-3 proteins significantly increased (P<0.05), and the expression levels of Bcl-2, p-PI3K, p-Akt and p-mTOR proteins significantly decreased (P<0.05).Conclusion Glycyrrhetinic Acid can inhibit the proliferation and promote the apoptosis of gastric cancer SGC7901 cells, which may be related to the inhibition of PI3K-Akt-mTOR signaling pathway.
[中图分类号]
R285.5
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