目的 研究小儿治哮灵片对幼年大鼠哮喘模型的干预作用及机制。方法 36只雄性Wistar幼年大鼠随机分为6组：对照组、模型组、醋酸地塞米松（阳性药，1 mg/kg）组和小儿治哮灵片低、中、高剂量（0.32、0.64、1.28 g/kg）组。除对照组外，其余各组以卵清蛋白（OVA）致敏、激发制备幼年大鼠哮喘模型。造模第8天开始ig给药，每天给药1次，给药至造模3周，对照组和模型组ig给予蒸馏水。记录各组大鼠引喘潜伏期并进行行为学评分；检测肺脏器指数；酶联免疫吸附法（ELISA）检测各组幼年大鼠血清中白细胞介素（IL）-4、IL-5、IL-6、免疫球蛋白E（IgE）、γ-干扰素（IFN-γ）、核转录因子（NF-κB）的水平以及肺泡灌洗液中IFN-γ水平。结果 与模型组比较，小儿治哮灵片高、中、低剂量组引喘潜伏期及哮喘行为评分均显著下降（P<0.05、0.01）；肺脏器指数显著下降（P<0.05）；血清IL-4、IL-5、IL-6、IgE、NF-κB水平均显著下降（P<0.01）；血清与肺泡灌洗液中IFN-γ水平显著升高（P<0.01）。结论 小儿治哮灵片抗哮喘作用可能与降低炎性细胞因子分泌有关。

Objective To study the interventional effect and mechanism of Xiaoer Zhixiaoling Tablets on asthmatic model in juvenile rats. Methods Thirty six young male Wistar rats were randomly divided into six groups:control group, model group, dexamethasone acetate (positive drug, 1 mg/kg) group and low, medium and high dose (0.32, 0.64, 1.28 g/kg) groups of Xiaoerzhixiaoling Tablets. Expect the blank control group, the other groups of young rats were sensitized by egg albumin (OVA) and stimulated to prepare the rat model of asthma in juvenile rats. On the 8th day of modeling, drugs were ig given once a day until 3 weeks for modeling. The control group and model group were given distilled water. The incubation period of asthma was recorded and the behavior score was evaluated. The levels of IL-4、IL-5、IL-6、IgE、IFN-γ、NF-κB in serum of young rats and the level of IFN-γ in alveolar lavage were detected by enzyme-linked immunosorbent method. Results Compared with the model group, the asthma latency and asthma behavior scores in the high, middle and low dose groups of Xiaoer Zhixiaoling Tablets were significantly decreased (P<0.05 and 0.01), and the lung organ index was significantly decreased (P<0.05); levels of IL-4, IL-5, IL-6, IgE, NF-κB decreased in the highdose group, the medium-dose group and the low-dose group (P<0.01), and levels of IFN-γ in serum and alveolar lavage fluid increased (P<0.01). Conclusion The anti-asthma effect of Xiaoer Zhixiaoling Tablets; may be related to the decreased secretion of downstream inflammatory cytokines.

R285.5

校企合作项目“小儿治哮灵片安全性评价研究”资助