[关键词]
[摘要]
目的 观察人源脐带间充质干细胞(hUC-MSCs)对异体总淋巴细胞和不同淋巴细胞亚群增殖能力的影响,以及对细胞因子分泌的影响。方法 分离并培养hUC-MSCs及健康成人外周血淋巴细胞,以淋巴细胞为阴性对照,MTT法检测共培养(淋巴细胞:hUC-MSCs为1:0.5、1:1、1:5、1:10)3 d后淋巴细胞的增殖情况;流式细胞仪检测共培养体系中CD4+CD25+FOXP3+、CD3-CD16+CD56+、CD4+CD45RA+CCR7+的细胞比例;ELISA法检测白细胞介素(IL)-2、IL-4、IL-10、γ-干扰素(INF-γ)细胞因子分泌情况。结果 与阴性对照组比较,hUC-MSCs对淋巴细胞的增殖具有显著抑制作用(P<0.05),抑制效果随数量增加而增强;hUC-MSCs能够显著增加共培养体系中CD4+CD25+FOXP3+细胞比例(P<0.05),显著降低CD4+CD45RA+CCR7+细胞比例(P<0.05),对CD3-CD16+CD56+细胞比例无显著影响;hUC-MSCs能够显著降低IL-2、IL-4、INF-γ水平,显著增加IL-10的分泌水平(P<0.05)。结论 hUC-MSCs具有免疫抑制功能。
[Key word]
[Abstract]
Objective To observe the effects of human umbilical cord mesenchymal stem cells (hUC-MSCs) on the proliferation of allogeneic total lymphocytes and different lymphocyte subsets, as well as the secretion of cytokines. Methods hUC-MSCs and peripheral blood lymphocytes of healthy adults were isolated and cultured; MTT method was used to detect the proliferation of lymphocytes by after co-culture for 3 days; the proportion of CD4+CD25+FOXP3+, CD3-CD16+CD56+ and CD4+CD45RA+CCR7+ in the co-culture system was detected by flow cytometry; ELISA was used to detect the secretion of IL-2, IL-4, IL-10 and INF-γ. Results hUC-MSCs could significant inhibit the proliferation of T lymphocytes, and the anti-proliferative effect was enhanced with the increase of the number of lymphocytes; the proportion of CD4+CD25+FOXP3+ cells in co-culture system was increased by hUCMSCs (P<0.05); the proportion of CD4+CD45RA+CCR7+ cells showed a downward tendency, and the difference was statistically significant (P<0.05); however, there was no significant difference in CD3-CD16+CD56+ cells. hUC-MSCs could reduce the levels of IL-2, IL-4 and INF-γ, but increase the secretion level of IL-10, and the difference was statistically significant (P<0.05). Conclusion HUC-MSCs had immunosuppressive function.
[中图分类号]
R329
[基金项目]
国家重点研发计划“干细胞及转化研究”重点专项(2019YFA0112100);天津市科技计划项目(17ZXXYSY00020)