目的 研究甘草酸铵对特应性皮炎（AD）小鼠IL-33/ST2通路及肥大细胞的活化情况的影响。方法 72只ICR小鼠（雌雄各半）随机均分为对照组、模型组及甘草酸铵低、中、高剂量（25、50、100 mg/kg）和泼尼松龙（阳性药，60 mg/kg）组。除对照组外，以丙酮-DNCB为致敏源构建AD小鼠模型，建模后各组ip相应药物，对照组和模型组ip生理盐水。记录小鼠夜间搔抓次数；甲苯胺蓝患处皮肤组织染色法观察肥大细胞活化情况；ELISA法检测白细胞介素（IL）-33和ST2血清学水平；实时荧光定量PCR（qRT-PCR）和Weston blotting法检测皮肤组织中的IL-33和ST2 mRNA和蛋白水平。结果 与对照组比较，模型组小鼠的搔抓次数显著增多（P<0.05），肥大细胞密度显著升高（P<0.05），IL-33和ST2的血清学水平、皮肤组织的转录水平和蛋白表达水平均显著升高（P<0.05）；与模型组比较，泼尼松龙组和甘草酸铵低、中、高剂量组的搔抓次数和肥大细胞密度分别显著减少和降低（P<0.05），IL-33、ST2的血清学水平、皮肤组织mRNA和蛋白表达均显著降低（P<0.05）。结论 甘草酸铵能够明显抑制AD模型小鼠IL-33和ST2的血清学水平、皮肤组织中的转录水平和蛋白表达，降低了肥大细胞的活化程度，从而减轻AD的瘙痒症状。

Objective To investigate the effect of ammonium glycyrrhizinate on the IL-33/ST2 pathway and the activation of mast cells in atopic dermatitis (AD) mice. Methods Tatolly 72 ICR mice (male and female) were randomly divided into control group, model group, ammonium glycyrrhizinate low, medium, high dose (25, 50, and 100 mg/kg) group, and bonisonone (positive drug, 60 mg/kg) group. In addition to control group, acetone-DNCB was used as the sensitization source to construct the AD mouse model. After the modeling, normal saline was ip into the abdomen of control and the model group. The corresponding drugs were ip to mice of each group. The number of scratches were count at night. The activation of mast cells in the skin tissue was observed by skin tissuetoluidine stained. The serum levels of IL-33 and ST2 were detected by ELISA; the mRNA and protein levels of IL-33 and ST2 in skin tissue were detected by real-time fluorescent quantitative PCR (qRT-PCR) and Weston blotting. Results Compared with control group, the scratching times of mice in model group increased significantly (P<0.05), mast cell density increased significantly (P<0.05), serum, transcription and protein expression levels of IL-33 and ST2 were significantly increased in the model group (P<0.05). Compared with model group, scratching times and mast cell density were significantly reduced in the bonisonone group and the ammonium glycyrrhizinate low, medium and high groups, respectively (P<0.05). The serological level, mRNA and protein expression of IL-33 and ST2 were significantly reduced (P<0.05). Conclusion Ammonium glycyrrhizinate can significantly inhibit the transcription level and protein expression of IL-33 and ST2 in serum and tissues of AD model mice, reduce the activation degree of mast cells, and thus relieve the itch symptoms of AD.

R965

南充市市校战略合作科技项目（18SXHZ0133）