目的 采用网络药理学方法对加味逍遥散治疗气滞痰阻型桥本氏甲状腺炎（HT）作用机制进行研究。方法 通过TCMSP、TCMID、TCMIP数据库收集筛选加味逍遥散当归、茯苓、白芍、白术、柴胡、半夏、厚朴、紫苏叶8味中药的主要化学成分并通过PubChem数据库收集各化合物化学式结构。分别通过Swiss Target Prediction、GeneCards、TCMIP数据库收集中药、疾病、证型的靶点，通过Bioinformatics Gent韦恩图收集“中药-疾病-证型”交互靶点，并分别通过STRING、WebGestal数据库对交互靶点进行PPI、GO、KEGG富集分析。结果 加味逍遥散有效活性成分：当归2个，茯苓15个，白芍13个，白术7个，柴胡17个，半夏13个，厚朴2个，紫苏叶14个；“中药-疾病-证型”交互靶点共43个；PPI结果显示，IL-6、VEGFA、AKT1、CASP3、TNF、HRAS、PTGS2等蛋白-蛋白相互作用关系最强；生物过程（BP）富集246个，细胞成分（CC）富集70个，分子功能（MF）富集12个类别、KEGG富集105条信号通路。结论 整合文献报道及网络药理学结果，加味逍遥散可能通过维持Th1/Th2平衡，调节炎症因子——白介素-6（IL-6）、肿瘤坏死因子（TNF）-α、TNF-β表达，通过HIF信号通路介导血管内皮生长因子（VEGF）在甲状腺细胞中广泛表达，通过VEGF信号通路参与调控甲状腺组织中血管新生的生理病理过程，在气滞痰阻型HT中发挥作用，同时预测加味逍遥散可能在HT转化成甲状腺乳头状癌（PTC）中发挥作用。

Objective To study the mechanism of Jiawei Xiaoyao Powder in the treatment of Hashimoto's thyroiditis (Hashimoto's thyroiditis, HT) with qi stagnation and phlegm stagnation. Methods The main chemical constituents of Jiawei Xiaoyao Powder Angelica sinensis, Poria cocos, Paeonia lactiflora Pall, Atractylodes macrocephala, Bupleurum chinense, Pinellia ternata, Magnolia officinalis, and Leaf of Perilla frutescens were collected by TCMSP, TCMID and TCMIP database, and the chemical formula structures of each compound were collected by PubChem database. The targets of traditional Chinese medicine, disease and syndrome type were collected by Swiss Target Prediction, GeneCards and TCMIP database respectively, and the interactive targets of "traditional Chinese medicine-disease-syndrome type" were collected by Bioinformatics Gent Wayne diagram, and the interactive targets were analyzed by PPI, GO and KEGG enrichment analysis through STRING and WebGestal database respectively, so as to clarify the mechanism of Jiawei Xiaoyao Powder in the treatment of HT with qi stagnation and phlegm stagnation. Results The active components of Jiawei Xiaoyao Powder were Angelica sinensis (2), Poria cocos (15), Paeonia lactiflora Pall (13), Atractylodes macrocephala (7), Bupleurum chinense (17), Pinellia ternata (13), Magnolia officinalis (2), Leaf of Perilla frutescens (14), 43 "traditional Chinese medicine-disease-syndrome" interaction targets, PPI results showed that IL6, VEGFA, AKT1, CASP3, TNF, HRAS, PTGS2 and other protein-protein interactions were the strongest. BP enriches 246 biological processes, CC enriches 70 cellular components, MF enriches 12 molecular functional classes, and KEGG enriches 105 signal pathways. Conclusion Jiawei Xiaoyao Powder may play a role in the pathogenesis of HT by maintaining the balance of Th1/Th2, regulating the expression of inflammatory factors (IL6, TNF-α, TNF-β), mediating the expression of VEGF molecules in thyroid cells through HIF signal pathway, and regulating the physiological and pathological process of angiogenesis in thyroid tissue through VEGF signal pathway. At the same time, it is predicted that Jiawei Xiaoyao Powder may play a role in the transformation of HT into PTC, which provides a direction for the follow-up study of the prognosis and treatment of HT complicated with PTC.

R285.5

湖南省卫生计生委科研计划课题项目（B20180739）；湖南省大学生研究性学习和创新型试验计划（2018425）；湖南中医药大学大学生创新型项目（201970）