目的 应用Micro-CT技术观察淫羊藿苷和朝藿定C对糖皮质激素性骨质疏松症（GIOP）小鼠模型骨组织骨量、骨微结构的影响。方法 8周龄C57/BL6雄性小鼠随机分为4组：对照组、模型组、淫羊藿苷（200 mg/kg）组和朝藿定C（200 mg/kg）组，除对照组外，其他3组小鼠im地塞米松5 mg/kg，0.125 mg/次，每周3次，制备GIOP模型，对照组im等体积的生理盐水，造模同时，每天ig给药1次，连续60 d后取材，采用micro-CT方法对胫骨近端骨微结构进行三维分析；HE染色病理切片观察胫骨近端骨组织病理形态。结果 骨量参数：与对照组比较，模型组骨矿物质含量（BMC）、骨矿物质密度（BMD）、组织矿物含量（TMC）、组织矿物质密度（TMD）均显著下降（P<0.05、0.01）；与模型组比较，淫羊藿苷组、朝藿定C组BMC、BMD、TMC、TMD指标均显著提高（P<0.05）；其中朝藿定C组各指标均较淫羊藿苷组显著升高（P<0.05）。与对照组比较，模型组相对骨体积（BV/TV）、骨小梁数量（Tb.N）、骨小梁厚度（Tb.Th）指标均显著下降（P<0.05），骨小梁分离度（Tb.Sp）、结构模型指数（SMI）均显著提高（P<0.05）；与模型组比较，淫羊藿苷、朝藿定C组BV/TV、Tb.N、Tb.Th显著升高，Tb.Sp、SMI显著下降（P<0.05），其中朝藿定C组各指标均较淫羊藿苷组改善显著（P<0.05）。HE染色病理切片示，模型组骨小梁数目明显减少，稀疏断裂，大部分不能连接成网状，骨髓腔明显增大，骨小梁结构出现较大的空白区域；淫羊藿苷及朝藿定C组小鼠骨小梁明显宽厚，数目也显著增加，骨小梁断裂较少，骨小梁光滑，接近对照组，其中朝藿定C组增加较淫羊藿苷组明显。结论 地塞米松诱导的骨质疏松小鼠模型成功建立，朝藿定C和淫羊藿苷抗骨质疏松活性明显，主要通过增加骨量和改善骨小梁微结构来最终提高骨强度，其中朝藿定C抗骨松作用更强；Micro-CT技术与传统的检测方法相比，在中药干预GIOP骨微结构参数分析上具有便捷、高效，经济，图像多维、全面，准确的优势。

Objective Micro-CT technique was used to observe the effects of icariin and epimedin C on bone mass and bone microstructure in the mouse model of glucocorticoid osteoporosis (GIOP). Methods Tatolly 40 C57/BL6 male mice of 8-week-old were randomly divided into four groups:control group, model group, icariin (200 mg/kg) group, and epimedin C (200 mg/kg) group. Except the control group, the other three groups were injected with dexamethasone 5 mg/kg, 0.125mg/time, three times a week, and the control group was injected with physiological saline of equal volume. At the same time, the drugs were gave by gavage once a day, and the samples were taken after 60 days. The microstructure of the proximal tibia was analyzed using Micro-CT method. The histopathology of the proximal tibia was observed by HE staining. Results Bone mass parameters:Compared with the control group, the BMC, BMD, TMC, and TMD indexes of the model group decreased significantly (P<0.01). Compared with the model group, the BMC, BMD, TMC and TMD indexes of the icariin group and the epimedin C group increased significantly. The index of epimedin group C was higher than that of icariin group. Bone microstructure parameters:compared with the control group, the indexes of BV/TV, TB.N, TB.SP and SMI in the model group decreased significantly, TB.SP and SMI increased significantly (P<0.05). Compared with the model group, the indexes of BV/TV, TB.N and TB.Th in the icariin group and epimedin C group increased, and the indexes of TB.SP and SMI decreased significantly (P<0.05). After HE staining pathological section in the model group, the number of bone trabeculae was significantly reduced, with sparse and broken pattern; most of them could not be connected into a network; the bone marrow cavity was significantly increased; the bone trabeculae structure appeared a large blank area. In the icariin and epimedin C group, similar to the control group, the bone trabeculae were obviously wider and thicker; the number was also noticeably increased; the bone trabeculae were less broken; the bone trabeculae were smooth. Moreover, epimedin C group increased more obviously than the icariin group. Conclusion Dexamethasone induced osteoporosis mouse model was established successfully. The anti-osteoporosis activity of epimedin C and icariin was obvious, and bone mass and bone trabecular microstructure were significantly improved, leading to the improvement of bone strength. Among them, epimedin C had stronger anti-osteoporosis effect. Compared with the traditional detection method, Micro-CT technology was convenient and efficient in the evaluation of GIOP bone microstructure parameters of traditional Chinese medicines.

R285.5

湖北省自然科学基金资助项目（2018CFB695）；广东省应用植物学重点实验室开放课题（AB2018027）；中国科学院华南农业植物分了分析与遗传改良重点实验室开放课题（KF202002）