[关键词]
[摘要]
目的 观察复方丹参片及其拆方对冠心病心肌缺血大鼠模型的影响。方法 SD大鼠随机分为假手术组,模型组,复方丹参片高、低剂量(0.6、0.3 g/kg)组,单硝酸异山梨酯片(阳性药,6.9 mg/kg)组,丹参提取物(0.3 g/kg)组,三七粉(0.3 g/kg)组,ig给药,每日1次,预防给药7 d,末次给药1 h后结扎冠脉左前降支制备心肌缺血大鼠模型。检测大鼠结扎前及结扎后Ⅱ导心电图,观察ST段变化值;最后一次心电图检测后腹主动脉取血,ELISA法检测血清肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)、天门冬氨酸转氨酶(AST)、一氧化氮(NO)、一氧化氮合成酶(eNOS)、内皮素(ET)、血栓素(TXB2)、前列环素(6-keto-F1α)水平;取心脏,TTC染色检测心肌缺血率。结果 与对照组比较,模型组心电图ST段显著抬高,心肌缺血明显,血清心肌酶AST、LDH、CK-MB水平均显著升高,TXB2水平显著升高,6-keto-F1α水平显著降低,NO、eNOS水平显著降低,ET水平显著升高(P<0.01、0.001)。与模型组比较,复方丹参片高、低剂量组心电图ST段抬高显著改善,心肌缺血率显著降低,血清各生化指标均显著改善(P<0.05、0.01);丹参提取物组心电图ST段抬高,心肌缺血率,血清CK-MB、LDH、eNOS、ET、TXB2、6-keto-F1α水平均显著改善(P<0.05),AST和NO水平差异无统计学意义;三七粉组心电图ST段、血清CK-MB、ET、TXB2、6-keto-F1α显著改善(P<0.05),心肌缺血率和AST、LDH、NO、eNOS水平差异无统计学意义。结论 在改善冠心病大鼠心肌缺血方面,复方丹参片效果较单味药好,丹参提取物效果较三七粉效果较好。
[Key word]
[Abstract]
Objective To observe the effect of Compound Danshen Tablet and its disassembled formula on the rat model of myocardial ischemia of coronary heart disease. Methods SD rats were randomly divided into sham operation group, model group, high and low dose groups (0.6 g/kg, 0.3 g/kg) of Compound Danshen Tablet, positive drug group (isosorbide mononitrate 6.9 mg/ kg), Danshen Extract group (0.3 g/kg), Sanqi powder group (0.3 g/kg). Rats were continuous administrated by gavage once a day for 7 days. One hour after the last administration, the left anterior descending of coronary artery (LAD) of rat was ligated to prepare the model of myocardial ischemia. Before and after ligation, the ECG of rat was respectively measured at 5, 15, 30, 45, 60, 90, 120, 180 min and 24 h, and the change of ST segment was determined. After the last ECG, the blood was taken from abdominal aorta, then detecting the level of CK-MB, LDH, AST, NO and eNOS, ET, TXB2, and 6-keto-F1α by ELISA assay. And the heart was taken to detect the infarct area. Results Compared with control group, the ST segment of ECG in the model group was significantly elevated, myocardial ischemia was significant, serum myocardial enzyme AST, LDH, CK-MB levels were significantly increased, TXB2 level was significantly increased, 6-keto-f1 α level was significantly reduced, NO, eNOS level was significantly reduced, ET level was significantly increased (P<0.01, 0.001). Compared with the model group, the ECG on the high and low dose group of Compound Danshen Tablet was significantly improved, as well as the index of vasomotor factor. Furthermore, Compound Danshen Tablet could decrease the serum myocardial enzyme and the antithrombotic index, the difference was statistically significant (P<0.01). ST segment of ECG, CK-MB, LDH, eNOS, ET, TXB2, 6-keto-F1α in Danshen Extract group were significantly improved (P<0.05), while AST and NO had no significantly difference. The other formula, Sanqi could significantly improve the ST segment of ECG, CK-MB, ET, TXB2 and 6-keto-F1α (P<0.05), but AST, LDH, NO and eNOS were not significantly changed. Conclusion According to experimental results, Compound Danshen Tablet showed the best improvement on myocardial ischemia of coronary heart disease. Meanwhile, the effect of Danshen extract group was better than Sanqi power group.
[中图分类号]
R285.5
[基金项目]
广东省重点领域研发计划项目(2020B1111110002);国家中医药管理局中药标准化项目(ZYBZH-C-GD-05);天津市科技计划项目(15ZXXYSY00070)