目的 以细胞凋亡和应激平衡损伤为切入点探讨舒脑欣滴丸（SNX）对脑缺血损伤大鼠急性后期的保护作用。方法 采用大鼠大脑中动脉电凝法制备脑卒中模型，造模成功大鼠随机分为5组，模型组、脑心通胶囊0.5 g/kg组和SNX 13、26、52 mg/kg组以及，每组10只，另设假手术组10只。脑缺血后48 h开始ig给药，每天1次，连续2 d。试剂盒法检测血清血管活性物质内皮素（ET）、一氧化氮（NO）活性，测定血清氧化应激因子超氧化物歧化酶（SOD）/丙二醛（MDA）水平；TUNEL染色测定细胞凋亡率，免疫组化法测定细胞凋亡相关蛋白Bcl-2/Bax比值、以及Caspase-3蛋白表达变化。结果 与模型组比较，SNX能一定程度减少细胞凋亡率，且可不同程度降低凋亡蛋白平衡Bax/Bcl-2比值；显著降低Caspase-3促凋亡蛋白因子的表达（P<0.01、0.001）；可调节氧化/抗氧化平衡，显著提高SOD/MDA值（P<0.05），并显著增加血管活性物质NO/ET比值（P<0.01）。结论 SNX对大鼠脑缺血急性晚期损伤引起的细胞凋亡及应激平衡失衡具有一定的改善作用。

Objective With apoptosis and stress balance injury as the entry point, to study the therapeutic effect of Shunaoxin Dropping Pill (SNX)on acute late stage of cerebral ischemic injury in rats. Methods The middle cerebral artery injury model was established by the electrocoagulation to prepare acute late-stage model of stroke in rats. Rats were equally divided into five groups (i.e model group, three SNX groups with 13, 26, and 52 mg/kg, positive control groups with NaoXinTong Capsule with 10 rats in each group. In addition, 10 rats without electrocoagulation were arranged as Sham group. The drug was administered on the 2nd day after cerebral ischemia, Each group was treated with corresponding drugs once daily for 2d. Serum NO and ET were measured by chemical method and enzyme linked immunosorbent assays. The oxidative stress cytokines involving serum SOD, MDA were measured by chemical methods. Cell apoptosis rates was determined by TUNEL staining, and the expression of apoptosis-related protein Bcl-2/Bax and the expression of Caspase-3 were observed by immunohistochemistry. Results Compared with model group, the cell apoptosis rates and the the expression of apoptosis-related protein Bax/Bcl-2 and Caspase-3 were decreased in different degree treated with SNX. The oxidation/anti-oxidation balance can be adjusted by SNX administration, serum SOD/MDA and NO/ET were increased than the model group (P<0.05、0.01). Conclusion SNX showed therapeutic benefits on rats in acute late-stage of cerebral ischemia injury.The potential mechanism of may be related to balance status of oxidation and anti-oxidation,Vasoactive substance, Bax/Bcl-2 ratio, and alleviating the expression Caspase-3, ultimately inhibit cell apoptosis.

R965

天津市第二批特支计划青年拔尖人才基金（TJTZJHQNBJRC-2-7）