[关键词]
[摘要]
目的 阐明蝉花水提物及蝉花子实体复方对小鼠免疫功能的影响,探讨其调节免疫的作用机制。方法 BALB/c小鼠70只随机分为7组(n=10):对照组,蝉花水提物高、中、低剂量(0.300、0.150、0.075 g/kg)组,蝉花复方高、中、低剂量(1.2、0.6、0.3 g/kg)组,ig给药15 d后检测小鼠胸腺指数、脾脏指数,通过脾淋巴细胞转化实验、溶血空斑实验以及碳廓清实验,观察淋巴细胞转化率、巨噬细胞吞噬功能和血清溶血素形成情况,评价蝉花水提物及蝉花子实体复方的免疫调节作用。结果 与对照组比较,低、中剂量蝉花水提物,中剂量蝉花子实体复方均能明显增加小鼠免疫器官脾脏、胸腺指数(P<0.05);低剂量蝉花水提物,低、中、高剂量蝉花子实体复方均能促进淋巴细胞增殖反应(P<0.05);低剂量蝉花水提物,低、中剂量蝉花子实体复方均能提高巨噬细胞吞噬功能(P<0.05)。结论 蝉花水提物及蝉花子实体复方均可明显提高机体免疫力,且复方在调节免疫功能强度方面较单用蝉花水提物有一定优势,说明蝉花预防和治疗免疫功能失调相关疾病有较好的应用前景。
[Key word]
[Abstract]
Objective To study the effect of Cicada flower water extract (CFWE) and Cicada flower synnema compound (CFSC) on immune function in mice, and explore its mechanism of regulating immunity. Methods Seventy BALB/c mice were randomly divided into 7 groups (n=10):control group, high, medium, and low doses of CFWE (0.300, 0.150, 0.075 g/kg), high, medium, and low doses of CFSC (1.2, 0.6, 0.3 g/kg). The thymus and spleen index of the mice were measured 15d after ig. The spleen lymphocyte transformation test, hemolytic plaque test, and carbon clearance test were used to observe the lymphocyte transformation rate, macrophage phagocytosis function, and serum hemolysin formation, and fully evaluate immune regulation of CFWE and CFSC. Results Compared with the control group, low and middle dosage of CFWE and middle dosage of CFSC could increase thymus and spleen index (P<0.05); Low dosage of CFWE and all dosages of CFSC could promote lymphocyte proliferative response (P<0.05); Low dosage of CFWE and low and middle dosage of CFSC could improve the phagocytosis function of macrophagocyte (P<0.05). Conclusion The water extract of Cicada flower and the Cicada flower synnema compound could obviously improve the body's immunity, and the compound has certain advantages in regulating the intensity and dose of the immune function than using the water extract of Cicada flower alone. It shows that Cicada flower has broad application prospects in the prevention and treatment of diseases related to immune dysfunction.
[中图分类号]
R285.5;R965
[基金项目]
国家科技重大专项(2015ZX09501004)