[关键词]
[摘要]
目的 探究芹菜素对妊娠期糖尿病(GDM)大鼠胰岛素抵抗的影响,并初步探究可能的作用机制。方法 于大鼠妊娠第5天,一次性尾iv 35 mg/kg链脲佐菌素混悬液制备GDM模型大鼠,随机分为模型组及芹菜素低、中、高剂量(10、20、40 mg/kg)组和二甲双胍(阳性对照,20 mg/kg),另设置正常妊娠大鼠为对照组,每组均12只。检测大鼠空腹血糖(FBG)、胰岛素(FINS)水平、胰岛素抵抗指数(HOMA-IR);全自动生化检测血清三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白(HDL-C)水平;硫代巴比妥酸法检测血清丙二醛(MDA)水平;氮蓝四唑比色法检测血清超氧化物歧化酶(SOD)水平;HE染色观察胰岛组织病理形态学变化;半定量评分评估胰岛组织损伤情况;Western blotting法检测胰岛组织磷脂酰肌醇3激酶(PI3K)、p-PI3K、蛋白激酶B(Akt)、p-Akt蛋白表达。结果 与对照组比较,模型组FBG、FINS、HOMA-IR显著升高,血清TC、TG、HDL-C水平显著升高,SOD水平显著降低,MDA水平显著升高,胰岛组织病理学评分显著升高,胰岛组织PI3K、Akt蛋白磷酸化水平显著降低(P<0.05);与模型组比较,芹菜素低、中、高剂量组与阳性对照组FBG、FINS、HOMA-IR显著降低,血清TC、TG、HDL-C水平显著降低,SOD水平显著升高,MDA水平显著降低,胰岛组织病理学评分显著降低,胰岛组织PI3K、Akt蛋白磷酸化水平显著升高(P<0.05)。结论 芹菜素能够改善大鼠血糖、血脂,降低胰岛素抵抗、保护胰岛组织,其机制可能与激活PI3K/Akt信号通路有关。
[Key word]
[Abstract]
Objective To investigate the effect of apigenin on insulin resistance in gestational diabetes mellitus (GDM) rats, and to explore the possible mechanism. Methods On the 5th day of pregnancy, GDM model rats were prepared by tail iv 35 mg/kg streptozotocin suspension, GDM model rats were randomly divided into model group, apigenin low, medium and high dose (10, 20 and 40 mg/kg) group and metformin group (positive control, 20 mg/kg). Normal pregnant rats were set as control group, with 12 rats in each group. The levels of fasting blood glucose (FBG), insulin (FINS), insulin resistance index (HOMA-IR) and serum triglyceride (TG), total cholesterol (TC) and high density lipoprotein (HDL-C) were measured by automatic biochemical method, the level of malondialdehyde (MDA) was measured by thiobarbituric acid method, superoxide dismutase (SOD) was detected by azoblue tetrazole colorimetry, histopathological changes of islets were observed by HE staining, semi-quantitative scoring was used to evaluate islet tissue injury, and the expressions of phosphatidylinositol 3 kinase (PI3K), p-PI3K, protein kinase B (Akt) and p-Akt in islet tissues were detected by Western blotting. Results Compared with control group, FBG, FINS and HOMA-IR increased in GDM group, TC, TG and HDL-C increased, the level of SOD decreased, the level of MDA increased, the histopathological score of islets increased, and the phosphorylation levels of PI3K and Akt proteins in islet tissue decreased (P < 0.05). Compared with GDM group, FBG, FINS and HOMA-IR decreased in Apigenin low, medium and high dose groups and Metformin group, TC, TG and HDL-C decreased, the level of SOD increased, the level of MDA decreased, the histopathological score of islets decreased, and the phosphorylation levels of PI3K and Akt proteins in islet tissue increased (P < 0.05). Conclusions Apigenin can improve blood glucose and blood lipid, reduce insulin resistance and protect islet tissue. Its mechanism may be related to activation of PI3K /Akt signal pathway.
[中图分类号]
R965
[基金项目]
河北省邢台市科技计划项目(2018ZC154)